mProX™ Human SCN10A Stable Cell Line

Product Information

Target Protein

SCN10A

Target Family

Voltage Gated Sodium Channel

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Episodic Pain Syndrome; Sodium Channelopathy-Related Small Fiber Neuropathy

Gene ID

6336

UniProt ID

Q9Y5Y9

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The SCN10A gene is a member of a group of genes that give instructions on how to create sodium channels. One component of a sodium channel called NaV1.8-the alpha subunit-is made according to instructions from the SCN10A gene. In pain-transmitting nerve cells termed nociceptors, sodium channel NaV1.8 is present. The peripheral nervous system, which links the brain and spinal cord to cells that sense touch, smell, and pain, includes nociceptors. The primary function of nociceptors is to transmit pain signals. The dorsal root ganglion, a region of the spinal cord, houses the nociceptors' cell bodies, or centers. Extending from the cell bodies are fibers known as axons that travel throughout the body to collect sensory data. Along with nociceptors, NaV1.8 sodium channels have also been discovered in heart muscle cells. By regulating the flow of sodium ions, these channels probably contribute to the maintenance of a healthy heart rhythm. The customized SCN10A stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Sandra (Verified Customer)

What is the significance of SCN10A in episodic pain? Sep 12 2021

chat Sherry Smith (Creative Biolabs Scientific Support)

SCN10A have been linked to nociceptors to episodic pain. Sep 12 2021

chat Dorothy (Verified Customer)

Is NaV1.8 the same as SCN10A? Jan 13 2021

chat Sherry Smith (Creative Biolabs Scientific Support)

Yes, the SCN10A gene encodes NaV1.8 sodium channels. Jan 13 2021

Published Data

Fig.1 Expression of SCN10A mRNA and NaV1.8 α subunit protein in human kidney tissues.

SCN10A mRNA was detected using the RT-PCR technique in human kidney tissues and HEK293T. Sperm-extracted SCN10A mRNA served as a positive control. An internal control, the mRNA of the housekeeping gene ACTB, was employed in the analysis of a section of SCN10A mRNA encompassing exons 2-3.

Ref: Nettuwakul, Choochai, et al. "Loss-of-function mutations of SCN10A encoding NaV1. 8 α subunit of voltage-gated sodium channel in patients with human kidney stone disease." Scientific reports 8.1 (2018): 10453.

Pubmed: 29992996

DOI: 10.1038/s41598-018-28623-3

Research Highlights

There is a correlation between the common variation A1073 and heightened vulnerability to AF. Variants that affect the channel's functionality, both frequent and unusual, suggest that they have an impact on an individual's vulnerability to AF.
Jabbari, Javad, et al. "Common and rare variants in SCN10A modulate the risk of atrial fibrillation." Circulation: Cardiovascular Genetics 8.1 (2015): 64-73.
Pubmed: 25691686 DOI: 10.1161/HCG.0000000000000022

Electrocardiographic PR and QRS intervals are highly correlated with genetic variations at the SCN5A/SCN10A locus. The canonical cardiac sodium channel gene is SCN5A, but little is known about SCN10A's function in cardiac conduction.
Macri, Vincenzo, et al. "Common coding variants in SCN10A are associated with the Nav1. 8 late current and cardiac conduction." Circulation: Genomic and Precision Medicine 11.5 (2018): e001663.
Pubmed: 29752399 DOI: 10.1161/CIRCGEN.116.001663