mProX™ Human RXRA Stable Cell Line

Product Information

Target Protein

RXRA

Target Family

Retinoid X Receptor

Target Protein Species

Human

Host Cell Type

GC; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Cancer Research; Metabolic Research

Related Diseases

Pancreatic Adenocarcinoma; Bladder Urothelial Carcinoma

Gene ID

6256

UniProt ID

P19793

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The RXRA gene in humans codes for the nuclear receptor known as retinoid X receptor alpha (RXR-alpha). Nuclear receptors called retinoid X receptors (RXRs) and retinoic acid receptors (RARs) participate in retinoic acid-mediated gene activation, which mediates the biological effects of retinoids. By attaching as homodimers or heterodimers to particular regions in the promoters of target genes, these receptors control the transcription of the target genes. The protein that this gene encodes belongs to the superfamily of transcription factors that includes thyroid hormone and steroid receptors. The RXR-RAR heterodimers bind to a multiprotein complex that contains transcription corepressors when there is no ligand present. This complex causes chromatin condensation, transcriptional suppression, and histone deacetylation. Transcriptional activation results from the corepressors' separation from receptors upon ligand binding and their association with coactivators. The customized RXRA stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Paul

The RXRA KO cell line design is well-optimized, and the provided documentation is comprehensive. Apr 15 2020

chat Verified Customer

chat Rebecca

Most importantly, I would recommend this product to anyone who wants to research RXRA. Aug 13 2020

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 The expression of RXRa in GC cell lines.

Using Q-PCR, the expression of RXRa mRNA was measured in three GC cell lines and GES-1, a normal gastric cell line. Western blotting of the aforementioned cell lines revealed RXRa expression.

Ref: Ren, Hong-Yue, et al. "IL-1β induced RXRα overexpression through activation of NF-κB signaling in gastric carcinoma." Biomedicine & Pharmacotherapy 78 (2016): 329-334.

Pubmed: 26898458

DOI: 10.1016/j.biopha.2016.01.033

Research Highlights

Growing evidence points to calcium signaling as a major mechanism governing cellular senescence, a stable stop of cell proliferation that is essential for pathologic states including cancer, aging, wound healing, and embryonic development.
Ma, Xingjie, et al. "The nuclear receptor RXRA controls cellular senescence by regulating calcium signaling." Aging Cell 17.6 (2018): e12831.
Pubmed: 30216632 DOI: 10.1111/acel.12831

PPAR inhibition can reverse the proliferation of urothelium caused by mutant RXRA, indicating that PPARs are targetable drivers of bladder cancer.
Halstead, Angela M., et al. "Bladder-cancer-associated mutations in RXRA activate peroxisome proliferator-activated receptors to drive urothelial proliferation." Elife 6 (2017): e30862.
Pubmed: 29143738 DOI: 10.7554/eLife.30862