mProX™ Human RXFP4 Stable Cell Line

Product Information

Target Protein

RXFP4

Target Family

Relaxin Family

Target Protein Species

Human

Host Cell Type

NCI-H716; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Gene ID

339403

UniProt ID

Q8TDU9

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

RXFP4, sometimes referred to as GPCR142 or GPR100, is mainly found in peripheral tissues, with the colorectum exhibiting the highest expression levels. Although there have been some disputes, its endogenous ligand INSL5, which is released by the colonic L-cell, was initially recognized as an incretin. Their pattern of expression and the deterioration in glucose and fat regulation observed in mice lacking INSL5 or RXFP4 suggest that they are involved in energy metabolism. Additionally described as an orexigenic hormone, INSL5, RXFP4 has been linked to nasopharyngeal carcinoma, colorectal cancer, and colon motility. Because of their diverse physiological characteristics and shared expression pattern in vivo, RXFP4 and RXFP3 require subtype-specific agonists that will probably be useful in a variety of clinical applications. The customized RXFP4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Brady (Verified Customer)

Can I use the cell line to monitor downstream signaling? Oct 16 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

Yes, the cell line can be ued to monitor downstream signaling. Oct 16 2022

chat Matt (Verified Customer)

Can the RXFP4 stable cell line be used in drug discovery research? Apr 12 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

Yes, the RXFP4 stable cell line can be used in drug discovery research. Apr 12 2023

Published Data

Fig.1 RXFP4 is expressed in human L-cell model NCI-H716 cell line.

RT-qPCR utilizing TaqMan probes was used to analyze the expression of the genes RXFP4, INSL5, GLP1R, and GCG in human NCI-H716 L-cells.

Ref: Ang, Sheng Y., et al. "INSL5 activates multiple signalling pathways and regulates GLP-1 secretion in NCI-H716 cells." Journal of molecular endocrinology 60.3 (2018): 213-224.

Pubmed: 29535183

DOI: 10.1530/JME-17-0152

Research Highlights

Colonic enteroendocrine cells, which also house the unrelated hormones GLP-1 and PYY, store insulin-like peptide 5 (INSL5). It affects the RXFP4 receptor, which is part of the relaxin family. Enteric neurons in the colon express RXFP4, and it has been hypothesized that INSL5's influence on these cells may play a role in the regulation of colonic contractions.
Diwakarla, Shanti, et al. "Colokinetic effect of an insulin-like peptide 5-related agonist of the RXFP4 receptor." Neurogastroenterology & Motility 32.5 (2020): e13796.
Pubmed: 31989750 DOI: 10.1111/nmo.13796

According to these findings, RXFP4VMH neurons control food preference and intake, and hypothalamic RXFP4 signaling is a target for changing eating behavior.
Lewis, Jo E., et al. "Relaxin/insulin-like family peptide receptor 4 (Rxfp4) expressing hypothalamic neurons modulate food intake and preference in mice." Molecular Metabolism 66 (2022): 101604.
Pubmed: 36184065 DOI: 10.1016/j.molmet.2022.101604