mProX™ Human RXFP2 Stable Cell Line

Product Information

Target Protein

RXFP2

Target Family

Relaxin Family

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cancer Research; Reproductive Research

Related Diseases

Cryptorchidism; Testicular Cancer

Gene ID

122042

UniProt ID

Q8WXD0

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

In terms of binding ability, RXFP2 can bind to relaxin-2, relaxin-3, and INSL-3, with INSL-3 having the highest affinity. Both the RXFP2 knockout mouse phenotype and the INSL-3 knockout mouse phenotype exhibit cryptorchidism. The gubernaculum-distributed RXFP2 that INSL-3 binds to controls the embryonic period testicle drop. In contrast, INSL-3 is insufficient to mediate healthy testicular aging. Androgen is necessary for the regulation of the suspensory ligament in testicular degeneration. The INSL-3-RXFP2 system controls not only testicular decline in male animals but also follicular atresia and corpus luteum apoptosis in female animals. Female INSL-3 mutant mice showed increased follicular atresia and early corpus luteum death. The customized RXFP2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Elizabeth

They are an excellent investment because of their unparalleled performance and quality. Jul 21 2023

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chat David

The RXFP2 cell line showed good performance in our hands. It was reliable for most experiments, and the customer support team was helpful in troubleshooting. May 01 2023

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FAQ

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Published Data

Fig.1 RXFP1& RXFP2 mRNA and protein expressions in the hamstring muscles.

The ratio of RXFP1 and RXFP2 to β-actin, as well as the expression of these mRNAs and proteins in the homogenates of hamstring muscle. Proestrus and diestrus were the times of greatest RXFP1 and RXFP2 mRNA and protein expression, respectively.

Ref: Dehghan, Firouzeh, et al. "Changes in knee laxity and relaxin receptor isoforms expression (RXFP1/RXFP2) in the knee throughout estrous cycle phases in rodents." PLoS One 11.8 (2016): e0160984.

Pubmed: 27513858

DOI: 10.1371/journal.pone.0160984

Research Highlights

Boar meiotic and post-meiotic germ cells expressed RXFP2, which suggests that INSL3 functions as a survival/anti-apoptotic factor in maintaining sperm production. INSL3 neutralization enhanced germ cell death and decreased sperm output.
Sagata, Dai, et al. "The insulin-like factor 3 (INSL3)-receptor (RXFP2) network functions as a germ cell survival/anti-apoptotic factor in boar testes." Endocrinology 156.4 (2015): 1523-1539.
Pubmed: 25562614 DOI: 10.1210/en.2014-1473

There are three varieties of leucine-rich repeat-containing G protein-coupled receptors (LGRs): types A, B, and C. All of these receptors have a sizable extracellular domain (ECD) made up of the structural motif leucine-rich repeat (LRR). The ECD binds the hormone or ligand in the LGRs, typically via the LRRs, which leads to activation and signaling at the end.
Petrie, Emma J., et al. "In a class of their own-RXFP1 and RXFP2 are unique members of the LGR family." Frontiers in endocrinology 6 (2015): 137.
Pubmed: 26441827 DOI: 10.3389/fendo.2015.00137