mProX™ Human RAF1 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- Kinase Cell Lines
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Published Data
Fig.1 The clonogenicity and tumourigenicity of colorectal cancer (CRC) cells were compromised by the knockdown (KD) of RAF1.
The diminished sphere-forming capability of colonospheres (CR16, CRC29, and L145) with RAF1 knockdown was evidenced by the clonogenic assay, with statistical significance denoted by *p<0.05, **p<0.01, and ***p<0.001.
Ref: Borovski, Tijana, et al. "Inhibition of RAF1 kinase activity restores apicobasal polarity and impairs tumour growth in human colorectal cancer." Gut 66.6 (2017): 1106-1115.
Pubmed: 27670374
DOI: 10.1136/gutjnl-2016-311547
Research Highlights
Donati, Michele. et al. "Spitz tumor with RAF1 fusion: A report of 3 cases." Annals of diagnostic pathology, 2023.
The study focused on Spitz tumors, a type of melanocytic neoplasms with distinct morphological features. These tumors are associated with various fusion kinases, including ALK, ROS1, NTRK1, NTRK2, NTRK3, MET, RET, BRAF, and MAP3K8 genes, or HRAS mutation. Recently, RAF1 fusions have also been identified in cutaneous melanocytic neoplasms such as conventional melanoma, congenital nevus, and BAP-1 inactivated tumors. The researchers report on three cases of Spitz neoplasms with a RAF1 fusion, including a previously reported CTDSPL::RAF1 fusion and two new fusions involving PPAP2B and ATP2B4 genes. These findings suggest that RAF1 fused melanocytic neoplasms may belong to a unique subgroup of Spitz tumors, with the RAF1 fusion serving as an oncogenic driver. Two of the cases were classified as Spitz nevus, while the third was initially diagnosed as Spitz melanoma due to a 9p21 homozygous deletion and positive sentinel lymph node biopsy.
Donati, Michele. et al. "Spitz tumor with RAF1 fusion: A report of 3 cases." Annals of diagnostic pathology, 2023.
Pubmed:
37856952
DOI:
10.1016/j.anndiagpath.2023.152215
Wang, Ran. et al. "Structural insight into the functions of Raf1 and Bsd2 in hexadecameric Rubisco assembly." Molecular plant, 2023.
The authors investigated the hexadecameric form I Rubisco enzyme, which is made up of eight large (RbcL) and eight small (RbcS) subunits. This enzyme is highly abundant on earth and plays a crucial role in agriculture. However, efforts to improve its catalytic efficiency through genetic manipulation have been hindered by its difficulty to fold and assemble correctly in foreign hosts or in vitro. The researchers successfully reconstituted Synechococcus sp. PCC6301 Rubisco in vitro using chaperonin and assembly factors from cyanobacteria and Arabidopsis thaliana. Cryo-EM structures of RbcL were obtained, showing the potential role of AtRaf1 and AtBsd2 in the assembly process.
Wang, Ran. et al. "Structural insight into the functions of Raf1 and Bsd2 in hexadecameric Rubisco assembly." Molecular plant, 2023.
Pubmed:
37853692
DOI:
10.1016/j.molp.2023.10.011
