mProX™ Human PTK6 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1042	Magic™ Human BRK(PTK6) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

PTK6

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

MCF7; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Ovary Serous Adenocarcinoma and Breast Cancer. Among its related pathways are Signaling by PTK6 and Regulation of activated PAK-2p34 by proteasome mediated degradation

Gene ID

5753

UniProt ID

Q13882

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The PTK6 gene in humans encodes the enzyme tyrosine-protein kinase 6. Tyrosine-protein kinase 6 is a cytoplasmic non-receptor protein kinase that is often referred to as breast tumor kinase (BRK). In epithelial tissues, it may have an intracellular signal transducer role. It has been demonstrated that the encoded protein experiences autophosphorylation. When this gene is overexpressed in mammary epithelial cells, the cells become more sensitive to epidermal growth factor, which causes a partial phenotypic transformation. While it is absent in healthy breast tissue, this gene has been found to express itself at low levels in certain breast cancers. The customized PTK6 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Linda

Its reliability and user-friendly design have simplified the process of PTK6 drug discovery in our lab. Feb 03 2023

chat Verified Customer

chat Melissa

I have used the PTK6 KO cell line extensively in my studies, and it consistently delivered outstanding performance. Aug 30 2023

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Generation of MMTV-PTK6 transgenic mice.

Expression of MMTV-PTK6 construct transfected into dexamethasone-stimulated NMuMG cells. The amounts of transgenic PTK6 protein (NMuMG +Tg) produced in human breast cancer cell lines MCF7, MDA-MB-231, and MDA-MB-453 are similar to each other. PTK6 in the MDA-MB-435 cell line was not found.

Ref: Peng, M., et al. "Protein tyrosine kinase 6 regulates mammary gland tumorigenesis in mouse models." Oncogenesis 2.12 (2013): e81-e81.

Pubmed: 24323291

DOI: 10.1038/oncsis.2013.43

Research Highlights

A negative result and elevated PTK6 PY342 are correlated with PTEN loss in human prostate carcinoma tissue microarrays. These findings imply that PTK6 activation encourages PTEN-induced invasive prostate cancer.
Wozniak, Darren J., et al. "PTEN is a protein phosphatase that targets active PTK6 and inhibits PTK6 oncogenic signaling in prostate cancer." Nature communications 8.1 (2017): 1508.
Pubmed: 29142193 DOI: 10.1038/s41467-017-01574-5

Comprehending the context-specific roles of PTK6 is crucial because, despite its ability to stimulate oncogenic signaling and cell survival following DNA damage, PTK6 expression in established tumors may preserve the epithelial phenotype, hence impeding tumor growth.
Mathur, Priya S., et al. "Kinase-dependent and-independent roles for PTK6 in colon cancer." Molecular Cancer Research 14.6 (2016): 563-573.
Pubmed: 26983689 DOI: 10.1158/1541-7786.MCR-15-0450