mProX™ Human PTH1R Stable Cell Line

Product Information

Target Family

Parathyroid Hormone Family

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1

Target Classification

Other Targets Drug Discovery Assays and Products

Target Research Area

Ocula Research;Metabolic Research

Related Diseases

Chondrodysplasia, Blomstrand Type;Metaphyseal Chondrodysplasia, Jansen Type

Gene ID

Human:5745

UniProt ID

Human:Q03431

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The Parathyroid hormone 1 receptor (PTH1R) is a key player in calcium homeostasis and bone metabolism. It's the primary receptor for parathyroid hormone (PTH) and parathyroid hormone-related protein (PTHrP). Activation of PTH1R regulates bone resorption and calcium reabsorption in the kidneys. Mutations in the PTH1R gene have been associated with various skeletal disorders. Moreover, PTH1R signaling plays a role in bone development, making it a potential therapeutic target for osteoporosis and other bone-related diseases.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Richard (Verified Customer)

How does PTH1R function in bone using the cAMP/Protein Kinase A pathway? Aug 04 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

PTH1R, when activated, can undergo endosomal translocation, similar to several hormones and other agonists. The cellular effects through PTH1R might be influenced when endosomal signaling is added to the traditional cAMP/Protein Kinase A pathway. Aug 04 2020

chat Deborah (Verified Customer)

Can PTH1R activation have therapeutic implications for epididymitis and orchitis? Jul 01 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

Yes, activation of PTH1R can alleviate symptoms of epididymitis and orchitis. This is achieved through the Gq and β-arrestin-1 pathways. Specifically, PTH1R in the epididymis and testis was found to be down-regulated in inflammation induced by mumps virus or lipopolysaccharide. Jul 01 2022

Published Data

Fig.1 In experiments where the intrinsic activity of PTH1R was elevated, it was observed that the application of PKA and PKC inhibitors resulted in a reduction of this activity.

In the experiment, cells transfected with PTH1R were exposed to H-89, GFX, or a combination of both, and the subsequent ratio of pERK to ERK1/2 was measured following a 5-minute stimulation period. The values obtained, representing this ratio, are indicative of the average results derived from a minimum of three separate trials. This method was employed to assess the cellular response under these specific conditions.

Ref: Candelario, Jose, Hesam Tavakoli, and Mirianas Chachisvilis. "PTH1 receptor is involved in mediating cellular response to long-chain polyunsaturated fatty acids." PLoS One 7.12 (2012): e52583.

Pubmed: 23300710

DOI: 10.1371/journal.pone.0052583

Research Highlights

Monica Reyes et al. "Substantially Delayed Maturation of Growth Plate Chondrocytes in "Humanized" PTH1R Mice with the H223R Mutation of Jansen's Disease." JBMR plus, 2023
Activating mutations in the PTH1R receptor leads to Jansen's metaphyseal chondrodysplasia (JMC), a rare condition characterized by growth plate issues, short stature, and elevated calcium levels unrelated to PTH. Previous transgenic JMC mouse models expressing the mutated H223R-PTH1R allele exhibited excessive bone mass when using specific promoters, but failed to fully replicate the human disease. A new "humanized" JMC mouse model was created, expressing the H223R-PTH1R allele via the endogenous mouse Pth1r promoter, resulting in small offspring with significant growth plate abnormalities. These mice may offer a better representation of the JMC phenotype and help evaluate potential treatments for this skeletal development and mineral ion homeostasis disorder.
Monica Reyes et al. "Substantially Delayed Maturation of Growth Plate Chondrocytes in "Humanized" PTH1R Mice with the H223R Mutation of Jansen's Disease." JBMR plus, 2023
Pubmed: 37808400 DOI: 10.1002/jbm4.10802

Md Azizul Haque et al. "Genome-Wide Association Studies for Body Conformation Traits in Korean Holstein Population." Animals : an open access journal from MDPI, 19 Sep. 2023
The objective of this study was to identify quantitative trait loci (QTL) and nearby candidate genes that influence body conformation traits. A population of 2329 Korean Holstein cattle was used for the study, with phenotypic data collected for 24 body conformation traits. All animals were genotyped using the 50 K Illumina bovine SNP chip. Through genome-wide association analysis, 24 genome-wide significant SNPs were identified for the body conformation traits. The selection of candidate genes was based on gene ontology (GO) terms and previous research on their impact on body conformation traits. Candidate genes such as KCNA1, RYBP, PTH1R, TMIE, and GNAI3 for body traits, ANGPT1 for rump traits, MALRD1, INHBA, and HOXA13 for feet and leg traits, and CDK1, RHOBTB1, and SLC17A1 for udder traits were identified. These findings provide insights into the genetic basis of body conformation traits and can guide future breeding strategies to enhance desirable traits in dairy cattle.
Md Azizul Haque et al. "Genome-Wide Association Studies for Body Conformation Traits in Korean Holstein Population." Animals : an open access journal from MDPI, 19 Sep. 2023
Pubmed: 37760364 DOI: 10.3390/ani13182964