mProX™ Human PTGER2 Stable Cell Line

Product Information

Target Protein

PTGER2

Target Family

Prostanoid Family

Target Protein Species

Human

Host Cell Type

OV3121; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

CNS Research; Inflammation Research

Related Diseases

Asthma; Nasal Polyps; And Aspirin Intolerance

Gene ID

5732

UniProt ID

P43116

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human gene PTGER2 encodes PTGER2, a 53 kDa prostaglandin receptor for prostaglandin E2 (PGE2). In humans, PTGER2 is widely distributed. The human small intestine, lung, uterus, thymus, cerebral cortex, corneal epithelium, and other organs all express it. The PTGER2 receptor performs a number of tasks. Rodents, cats, rhesus monkeys, and humans all saw a reduction in intraocular pressure when the PTGER2 receptor was topically given to their eyes. This effect appears to be caused, at least in part, by PTGER2. According to studies, female mice that have been genetically altered to be deficient in the PTGER2 gene have a mildly reduced rate of ovulation and a significantly reduced ability to become pregnant. Additionally, PTGER2 activation affects allergy and inflammatory responses. The PTGER2 receptor can also stimulate tumor growth. Additionally, following exposure to carcinogens, lung, breast, skin, and colon cancers are less common in PTGER2 gene knockout mice. The customized PTGER2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Victoria

Excellent service! Very reliable. Fast turnaround time. Nov 19 2022

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chat Joseph

I recomended PTGER2 Knock out cell line to my lab members. The delivery is much faster than I expected. Feb 19 2023

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FAQ

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Published Data

Fig.1 EP2 interacts with CTR in OV3121 cell line.

Expression of Ptger2 and Calcr mRNA in OV3121 cells. From OV3121 cells, total RNA was extracted. First strand cDNA was then created using reverse transcriptase (RT+) or without it (RT-), and RT-PCR was conducted.

Ref: Matsubara, Shin, et al. "Heterodimerization of the prostaglandin E2 receptor EP2 and the calcitonin receptor CTR." Plos one 12.11 (2017): e0187711.

Pubmed: 29095955

DOI: 10.1371/journal.pone.0187711

Research Highlights

The prostaglandin E2 receptor 2 (PTGER2) is found in the endometrium, and endometrial expansion is associated by PTGER2 gene expression. Cattle endometrial PTGER2 activation could promote the expression of VEGF, CTGF, TGF-β1, and IL-8 genes, leading to endometrial healing.
Gao, Long, et al. "PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle." Animal reproduction science 187 (2017): 54-63.
Pubmed: 29033117 DOI: 10.1016/j.anireprosci.2017.10.005

By controlling the methylation of prostaglandin E receptor 2 (PTGER2) and obstructing TGF-β signaling, HA-Cur dramatically reduced the fibrotic activities of myofibroblast in joint contracture in vitro. In addition to offering a molecular target for therapy during the etiology of joint contracture, this may offer a mechanism for the treatment of joint contracture.
Yu, Dongjie, et al. "Hyaluronic acid-curcumin conjugate suppresses the fibrotic functions of myofibroblasts from contractive joint by the PTGER2 demethylation." Regenerative Biomaterials 6.5 (2019): 269-277.
Pubmed: 31616564 DOI: 10.1093/rb/rbz016