mProX™ Human PROKR2 Stable Cell Line

Product Information

Target Protein

PROKR2

Target Family

Prokineticin Family

Target Protein Species

Human

Host Cell Type

COS-7; HeLa; CHO-K1; HEK293T

Target Classification

GPCR Cell Lines

Target Research Area

Cardiovascular Research; Inflammation Research; Reproductive Research

Related Diseases

Hypogonadotropic Hypogonadism;Kallmann Syndrome

Gene ID

128674

UniProt ID

Q8NFJ6

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

On the membrane of GnRH neurons, PROKR2 is a G protein-coupled receptor (GPCR) whose activation encourages GnRH production. About 5% of congenital hypogonadotropic hypogonadism with or without anosmia is caused by loss-of-function mutations in this gene. Contrary to other mRNA frameshift mutations that result in a stop-codon, the detected mutation, p.C242fsX305, shown to evade nonsense-mediated mRNA degradation. The results of an in-vitro test showed that while the wild-type receptor's activity was increased in a heterozygous state, the variant receptor did not exert any activity on its own. It's still unclear exactly how something works. Due to the shortened C-terminal region, hyperactive mutant-wild-type heterodimers can have a lower receptor internalization or a higher ligand affinity. The customized PROKR2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Taisei

We are quite happy with the knockout cells that you made. Mar 05 2022

chat Verified Customer

chat Nick

The PROKR2 cell line is an essential tool for anyone studying drug discovery. Jan 11 2023

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 RER1 is involved in retrograde transport of P290S PROKR2.

Evaluating the interactions between WT or P290S PROKR2 and the cargo receptors KDELR or RER1. An anti-HA antibody that was directed against PROKR2-3F3H, which was persistently expressed in HEK293T cells, was used for immunoprecipitation.

Ref: Song, Yong Bhum, et al. "Trafficking-defective mutant PROKR2 cycles between endoplasmic reticulum and Golgi to attenuate endoplasmic reticulum stress." Proceedings of the National Academy of Sciences 119.8 (2022): e2102248119.

Pubmed: 35173048

DOI: 10.1073/pnas.2102248119

Research Highlights

A G-protein-coupled receptor called PROKR2 that can bind both PROK1 and PROK2 is known as the prokineticin receptor 2. Patients with PROKR2 mutations frequently exhibit Kallmann Syndrome, anosmia/hyposmia, or hypogonadotropic hypogonadism.
Martinez-Mayer, Julian, and Maria Ines Perez-Millan. "Phenotypic and genotypic landscape of PROKR2 in neuroendocrine disorders." Frontiers in Endocrinology 14 (2023): 1132787.
Pubmed: 36843573 DOI: 10.3389/fendo.2023.1132787

Idiopathic hypogonadotropic hypogonadism (IHH) is a rare condition brought on by inadequate gonadotropin-releasing hormone synthesis, secretion, or action. A GPCR called PROKR2 is encoded by the prokineticin (PROK) receptor 2 (PROKR2) gene, which is the cause of IHH.
Zhao, Yaguang, et al. "PROKR2 mutations in idiopathic hypogonadotropic hypogonadism: selective disruption of the binding to a Gα-protein leads to biased signaling." The FASEB Journal 33.3 (2019): 4538-4546.
Pubmed: 30576231 DOI: 10.1096/fj.201801575R