mProX™ Human PROKR1 Stable Cell Line

Product Information

Target Protein

PROKR1

Target Family

Prokineticin Family

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293T

Target Classification

GPCR Cell Lines

Target Research Area

Cardiovascular Research; Inflammation Research

Related Diseases

Hirschsprung Disease; Kallmann Syndrome

Gene ID

10887

UniProt ID

Q8TCW9

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Adipose tissue and the cardiovascular system both benefit from prokineticin receptor 1 (Prokr1)'s improved metabolic profile; however, its effects on skeletal muscle have not been studied. We looked at the metabolic role of Prokr1 in the murine myoblast, satellite cells, and their developed myotubes. We examined the Prokr1 expression levels in both human skeletal muscle cell-derived myotubes and mouse skeletal muscle. In HEK293T cells that had PROKR1 overexpressed, prokineticin 2 (PROK2), a ligand of PROKR1, changed the mRNA levels of 578 genes and dose-dependently increased calcium mobilization. In skeletal muscle cells, PROKR1 stimulated Gq-mediated PI3K/AKT and MAPK/ERK signaling pathways, according to functional enrichment analysis of differentially expressed genes. In myotubes generated from C2C12 and satellite cells, Prokr1 dramatically stimulated the PI3K/AKT signaling pathway, regardless of the presence or absence of insulin. Glucose transporter 4 (GLUT4) was also helped enter the plasma membrane by Prokr1. Prokr1 improved insulin-stimulated AKT phosphorylation, GLUT4 translocation, and glucose uptake in myotubes that had developed insulin resistance due to palmitate. The customized PROKR1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Jacqueline (Verified Customer)

Is the receptor in my product full length? Feb 03 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

The amino acids that were included are indicated if an amino acid range is given. If there is no listed range of amino acids, the receptor's entire length was used. Feb 03 2023

chat Jean (Verified Customer)

Is transgene expression measured by flow cytometry? Aug 30 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

Our cell lines from which expression data was generated via flow cytometry analysis. Aug 30 2023

Published Data

Fig.1 Signaling pathways of PROKR1.

Expression analysis of PROKR1 overexpression in HEK293T cells, PROKR1 mRNA and protein in HEK293T cells, mock control with an empty vector, and blank control.

Ref: Mok, Jongsoo, et al. "Prokineticin receptor 1 ameliorates insulin resistance in skeletal muscle." The FASEB Journal 35.2 (2021): e21179.

Pubmed: 33184929

DOI: 10.1096/fj.202001641R

Research Highlights

The distribution of PROKR1 protein by CDVs made from genetically modified cells seems to be a successful procedure, and they show promise as an alternative treatment for muscular dystrophy.
Zhang, Chunjuan, et al. "PROKR1 delivery by cell-derived vesicles restores the myogenic potential of Prokr1-deficient C2C12 myoblasts." Nanomedicine: Nanotechnology, Biology and Medicine 37 (2021): 102448.
Pubmed: 34314870 DOI: 10.1016/j.nano.2021.102448

In order to identify the expression of PROK 1 and PROKR 1 in endometriosis-related eutopic and ectopic endometrium as well as normal control endometrium, quantitative real-time PCR (qPCR) and Western blotting were used. The development of endometriosis and its pathophysiology are influenced by PROK-1 and its receptors. TNF-α can stimulate angiogenesis by increasing PROK 1 expression.
Wu, Yahong, and Xianqing Wu. "Expression of PROK 1 and its receptor PROKR 1 in endometriosis and its clinical significance." Journal of Central South University. Medical Sciences 44.6 (2019): 621-627.
Pubmed: 31304922 DOI: 10.11817/j.issn.1672-7347.2019.06.003