mProX™ Human PROK2 Stable Cell Line

Product Information

Target Family

Other Targets

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;HeLa

Target Classification

Other Targets Drug Discovery Assays and Products

Target Research Area

Metabolic Research

Related Diseases

Hypogonadotropic Hypogonadism 4 With Or Without Anosmia; Kallmann Syndrome

Gene ID

Human:60675

UniProt ID

Human:Q9HC23

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Prokineticin 2 (Prok2) is a small protein expressed in a subpopulation of neurons in the suprachiasmatic nucleus (SCN), which is the primary circadian pacemaker in mammals. It has been implicated as a candidate output molecule from the SCN to control multiple circadian rhythms. In vivo recording of the circadian calcium rhythm in Prok2 neurons of the SCN has been achieved using Prok2-tTA knock-in mice expressing the tetracycline transactivator specifically in Prok2 neurons. The circadian Ca2+ rhythm in these neurons showed clear rhythms in both light-dark and constant dark conditions, with their peaks around midday. The hours of high Ca2+ coincided with the rest period of the behavioral rhythm, supporting the predicted function of Prok2 neurons in suppressing locomotor activity during both daytime and subjective daytime. Additionally, the application of a hybrid framework called Circadian Gene Regulatory Framework (CGRF) has been introduced to infer circadian gene regulatory relationships from gene expression data. This framework combines the fuzzy C-means clustering algorithm with dynamic time warping distance to efficiently identify gene clusters related to the target gene and determine the significance of genes within a specific cluster. The framework also uses a dynamic vector autoregressive method to analyze selected significant gene expression profiles and reveal directed causal regulatory relationships based on partial correlation. Furthermore, the potential role of Prok2 gene polymorphisms as predictors of methamphetamine use disorder risk and indicators of craving scale in the Chinese Han population has been investigated. The study identified a significant association between the PROK2 gene and both MUD risk susceptibility and craving scale, providing insights into the underlying mechanisms of METH dependence. Lastly, chronic social stress has been shown to blunt core body temperature and molecular rhythms of Rbm3 and Cirbp in the mouse lateral habenula. The study found that chronic social stress dampened temperature amplitudes and fragmented the interdaily stability of temperature rhythms, with recovery observed in stress-resilient mice. The expression of thermosensitive genes Rbm3 and Cirbp in the lateral habenula was also blunted after stress exposure but recovered later.

Protocols

Please visit our protocols page.

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FAQ

chat Taylor Davis (Verified Customer)

What is the role of PROK2 in inflammatory processes? May 24 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

PROK2 can induce a pro-inflammatory response in certain conditions, such as in murine fetal membranes, although its role in inducing preterm delivery is not clear. May 24 2023

chat Casey Garcia (Verified Customer)

How does PROK2 contribute to neurological disorders? Oct 01 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

PROK2 signaling is implicated in neurological processes, including the migration of olfactory bulb interneurons, and may be involved in disorders like Alzheimer's disease. Oct 01 2023

Published Data

Fig.1 The impact of PROK2 knockdown on cell viability in HeLa cells of human cervical cancer was investigated.

The protein expression of PROK2 was assessed in HeLa cells transfected with either shLuc or shPROK2. Cell viability at 24 h and 48 h after seeding was measured through a cell viability assay in both shLuc- and shPROK2-transfected HeLa cells.

Ref: Wu, Min-Hua, et al. "Silencing PROK2 inhibits invasion of human cervical cancer cells by targeting MMP15 expression." International Journal of Molecular Sciences 21.17 (2020): 6391.

Pubmed: 32887509

DOI: 10.3390/ijms21176391

Research Highlights

Onodera, Kaito. et al. "In vivo recording of the circadian calcium rhythm in Prokineticin 2 neurons of the suprachiasmatic nucleus." Scientific reports, 2023.
The study of Prokineticin 2 (Prok2) centers around a small protein found in a specific group of neurons within the suprachiasmatic nucleus (SCN) - the primary circadian pacemaker in mammals. Researchers have suggested that Prok2 may act as an output molecule from the SCN, playing a role in regulating various circadian rhythms. To further investigate this function, a team has successfully created Prok2-tTA knock-in mice with tetracycline transactivator (tTA) specifically expressed in Prok2-producing neurons. This new animal model allowed for in vivo recording of calcium levels, providing valuable insights into the function of Prok2 neurons.
Onodera, Kaito. et al. "In vivo recording of the circadian calcium rhythm in Prokineticin 2 neurons of the suprachiasmatic nucleus." Scientific reports, 2023.
Pubmed: 37813987 DOI: 10.1038/s41598-023-44282-5

Hu, Shuwen. et al. "Inferring circadian gene regulatory relationships from gene expression data with a hybrid framework." BMC bioinformatics, 2023.
The mammalian body's central biological clock plays a crucial role in regulating various physiological functions, such as sleep, metabolism, and immune system activity. To fully comprehend the underlying mechanisms of these cellular processes, it is essential to understand the relationships between different genes and their regulation. However, inferring circadian gene regulatory relationships solely based on correlation may not reveal the true causation. Additionally, the analysis of gene expression data can be challenging due to the high number of dimensions and limited number of observations.
Hu, Shuwen. et al. "Inferring circadian gene regulatory relationships from gene expression data with a hybrid framework." BMC bioinformatics, 2023.
Pubmed: 37752445 DOI: 10.1186/s12859-023-05458-y