mProX™ Human PRKCI Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX449	Magic™ Human PKCι(PRKCI) in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;U2OS

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research;CNS Research

Related Diseases

Lung Cancer; Hepatocellular Carcinoma

Gene ID

Human:5584

UniProt ID

Human:P41743

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

PRKCI, which stands for protein kinase C iota, has various applications in different fields. In the field of mental health, PRKCI has been studied in relation to suicide. Research has shown that deaths by suicide are significantly increased during the week of the full moon, and PRKCI is one of the biomarkers predictive of death by suicide during this time. PRKCI is also associated with the peak hour of suicide and the peak month of suicide. In the field of cancer, PRKCI has been found to play a role in human cancer, particularly in pancreatic cancer and hepatocellular carcinoma. In pancreatic cancer, PRKCI is one of the differentially expressed genes that can be used for prognostic assessments. In hepatocellular carcinoma, a specific single nucleotide polymorphism (SNP) in PRKCI has been associated with an increased risk of HCC in the Pakistani population. Finally, PRKCI has also been studied in the context of hybridization in mammals, specifically in the russet ground squirrel. The study found that PRKCI contributes to the genetic variability of the squirrel and highlighted the importance of conservation measures for this species. Overall, PRKCI has implications in mental health, cancer, and evolutionary biology.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Cameron Williams (Verified Customer)

What is the impact of PRKCI on gastric cancer cell proliferation and invasion? Jul 28 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

Circ-PRKCI promotes cell proliferation and invasion in gastric cancer by binding to microRNA-545. Jul 28 2022

chat Cameron Miller (Verified Customer)

How does PRKCI mediate radiosensitivity in cervical cancer? Nov 18 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

High PRKCI expression decreases survival in cervical cancer and regulates radiosensitivity by modulating the Hedgehog/GLI1 pathway. Nov 18 2021

Published Data

Fig.1 Autophagosome attenuation is achieved through overexpression of PRKCI.

In the upper panel, the endogenous LC3B-II protein levels were assessed in U2OS cells following exposure to CQ (25 μM, 4 h), RAPA (100 nM, 4 h), or TG (1 μM, 6 h). In the lower panel, representative confocal microscopy images of GFP-LC3B distribution were captured in U2OS cells that had been transfected with plasmids and subjected to treatment as described in the upper panel.

Ref: Qu, Liujing, et al. "PRKCI negatively regulates autophagy via PIK3CA/AKT-MTOR signaling." Biochemical and biophysical research communications 470.2 (2016): 306-312.

Pubmed: 26792725

DOI: 10.1016/j.bbrc.2016.01.059

Research Highlights

Bhagar, R. et al. "Temporal effects on death by suicide: empirical evidence and possible molecular correlates." Discover mental health, 2023.
The present study aims to empirically investigate the purported correlation between full moons and mental health issues, specifically suicide rates. A dataset of 776 pre-COVID suicides from the Marion County Coroner's Office was examined. Results suggest a significant increase in suicides during the week of a full moon (p=0.037), with older individuals (age,â•55) showing a stronger effect (p=0.019). Furthermore, a subset of 45 subjects had blood samples analyzed, revealing potential molecular mechanisms for suicide. Additionally, the study observed a higher number of deaths by suicide between 3-4 pm (p=0.035) and during the month of September (p=0.09). Previous studies on blood biomarkers for suicidality (n=154) were also considered.
Bhagar, R. et al. "Temporal effects on death by suicide: empirical evidence and possible molecular correlates." Discover mental health, 2023.
Pubmed: 37861857 DOI: 10.1007/s44192-023-00035-4

Yu, Liqing. et al. "Circ_0067934: a circular RNA with roles in human cancer." Human cell, 2023.
Circular RNA (circRNA) is a type of non-coding RNA formed through reverse splicing from pre-mRNA. It lacks a 5' cap structure and possesses a poly-adenylated tail at the 3' end. Advances in RNA sequencing and bioinformatics have unveiled the clinical significance of circRNAs. Circ_0067934, a 170-nucleotide RNA molecule on chromosome 3q26.2, results from exons 15 and 16 reverse splicing in PRKCI. Studies have shown varied circ_0067934 levels in different tumors, correlating with tumor size, TNM stage, and prognosis. Its impact on cancer cell behaviors, such as proliferation and invasion, is influenced by interactions with multiple microRNAs and modulation of signaling pathways, like Wnt/β-catenin and PI3K/AKT. These findings pave the way for circ_0067934's potential applications in clinical diagnosis, therapy, and prognosis evaluation across various cancers.
Yu, Liqing. et al. "Circ_0067934: a circular RNA with roles in human cancer." Human cell, 2023.
Pubmed: 37592109 DOI: 10.1007/s13577-023-00962-y