mProX™ Human PRKAA1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1029	Magic™ Human AMPKα1/β1/γ1 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

PRKAA1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

C-4I; HTB-35; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research; Cardiovascular Research

Related Diseases

Wolff-Parkinson-White Syndrome and Myotonia. Among its related pathways are Glucose / Energy Metabolism and PI3K-Akt signaling pathway

Gene ID

5562

UniProt ID

Q13131

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The Human PRKAA1 gene encodes the catalytic subunit alpha-1 of 5'-AMP-activated protein kinase. The serine/threonine protein kinase family includes the protein that this gene encodes. It is the 5'-prime-AMP-activated protein kinase's catalytic subunit (AMPK). All eukaryotic cells have the conserved cellular energy sensor AMPK. Stimuli that raise the cellular AMP/ATP ratio stimulate the kinase activity of AMPK. By phosphorylating several important metabolic enzymes, AMPK controls their activity. It prevents ATP-consuming biosynthesis pathways from being used, shielding cells against stressors that lead to ATP depletion. Different transcript variants with alternative splicing that encode different isoforms have been found. The customized PRKAA1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Brady

If you are looking for a good value PRKAA1 cell line for drug discovery, this is the one. Oct 16 2022

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chat Matt

This PRKAA1 cell line is highly selective. Its performance has been outstanding, demonstrating reliable and consistent binding. Apr 12 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Characteristics of cervical carcinoma cell lines.

AMPKα protein expression and activation were assessed via Western Blot analysis. Representative findings from three separate evaluations are displayed in membrane images. Densitometry between the reference gene and the studied gene is the basis for the quantitative results displayed on bar graphs.

Ref: Konieczny, Paweł, et al. "Impact of AMPK on cervical carcinoma progression and metastasis." Cell Death & Disease 14.1 (2023): 43.

Pubmed: 36658117

DOI: 10.1038/s41419-023-05583-9

Research Highlights

Genetic polymorphisms in PRKAA1, the catalytic subunit of AMP-activated protein kinase (AMPK), have been linked to an increased risk of gastric cancer. AMPK is a crucial enzyme that regulates cellular energy metabolism through phosphorylation.
Zhang, Yangmei, et al. "PRKAA1 promotes proliferation and inhibits apoptosis of gastric cancer cells through activating JNK1 and Akt pathways." Oncology research 28.3 (2020): 213.
Pubmed: 31558185 DOI: 10.3727/096504019X15668125347026

Large-scale, carefully planned case-control studies are needed to examine the association in order to validate the risk that the current meta-analysis found, particularly among non-Asian ethnicity.
Jiang, You, et al. "Association between PRKAA1 rs13361707 T> C polymorphism and gastric cancer risk: Evidence based on a meta-analysis." Medicine 97.14 (2018).
Pubmed: 29620653 DOI: 10.1097/MD.0000000000010302