mProX™ Human PPARG Stable Cell Line

Product Information

Target Protein

PPARG

Target Family

Peroxisome Proliferator-activated Receptor

Target Protein Species

Human

Host Cell Type

HepG2; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Diabetes Research; Metabolic Research

Related Diseases

Lipodystrophy

Gene ID

5468

UniProt ID

P37231

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Encoded by the PPARG gene in humans, peroxisome proliferator-activated receptor gamma (PPAR-γ or PPARG) is a type II nuclear receptor that functions as a transcription factor. PPARG controls the metabolism of glucose and the storage of fatty acids. PPARG-activated genes promote fat cells' adipogenesis and lipid absorption. PPARG is a master regulator of adipocyte development, as demonstrated by the absence of adipose tissue in PPARG knockout mice. PPARG improves fatty acid storage in fat cells (lowering lipotoxicity), promotes adiponectin release from fat cells, induces FGF21, and upregulates the CD38 enzyme to boost nicotinic acid adenine dinucleotide phosphate synthesis. All of these mechanisms increase insulin sensitivity. The customized PPARG stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The PPARG overexpression cell line has not disappointed. It consistently delivers accurate results, making my work much more impactful. Mar 22 2021

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The protocol of human PPARG cell line was straightforward, and the results were consistent. Sep 07 2022

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FAQ

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Published Data

Fig.1 Establishment of Stable Cell Lines to Detect PPARγ Activators.

Each cell line that was grown for one day with or without Tet had its nuclear extracts submitted to SDS-PAGE and immunoblotting.

Ref: Tachibana, Keisuke, et al. "Development of a ligand screening tool using full-length human peroxisome proliferator-activated receptor-expressing cell lines to ameliorate metabolic syndrome." Chemical and Pharmaceutical Bulletin 67.3 (2019): 199-202.

Pubmed: 30827999

DOI: 10.1248/cpb.c18-00627

Research Highlights

Six variations were classified as harmful by the classifier when it was applied to 55 novel missense variants found by population-based and clinical sequencing; these were then confirmed through single-variant testing. When missense variations in disease-related genes are found, saturation mutagenesis and future experimental characterization can help with the prompt diagnostic interpretation of the variation.
Majithia, Amit R., et al. "Prospective functional classification of all possible missense variants in PPARG." Nature genetics 48.12 (2016): 1570-1575.
Pubmed: 27749844 DOI: 10.1038/ng.3700

In the past ten years, the PPAR nuclear receptor family-which is made up of three distinct isoforms-has grown significantly in importance. (PPARα, PPARβ/δ, and PPAR ϒ). The steroid receptor superfamily, which includes those nuclear receptors, is involved in vital metabolic and life-sustaining processes.
Bandera Merchan, Borja, Francisco José Tinahones, and Manuel Macías-González. "Commonalities in the association between PPARG and vitamin D related with obesity and carcinogenesis." PPAR research 2016 (2016).
Pubmed: 27579030 DOI: 10.1155/2016/2308249