mProX™ Human PPARA Stable Cell Line

Product Information

Target Protein

PPARA

Target Family

Peroxisome Proliferator-activated Receptor

Target Protein Species

Human

Host Cell Type

HepG2; Huh-7; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Digestive and Renal Research; Metabolic Research

Related Diseases

Fatty Liver Disease; Lipid Metabolism Disorder

Gene ID

5465

UniProt ID

Q07869

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The PPARA gene encodes the nuclear receptor protein known as peroxisome proliferator-activated receptor alpha (PPAR-α), which functions as a transcription factor in humans. It was the original member of the PPAR family and was shown to be the nuclear receptor for a broad range of hepatocarcinogens in rodents that cause peroxisomes to proliferate. One of the main transcription factors controlling lipid metabolism in the liver is PPAR-α, which is activated by free fatty acids. Energy deprivation triggers the activation of PPAR-alpha, which is essential for the process of ketogenesis, a crucial adaptive response to extended fasting. By upregulating genes involved in fatty acid transport, fatty acid binding and activation, peroxisomal and other membrane transport, and fatty acid catabolism, PPAR-alpha activation, and peroxisomal and mitochondrial fatty acid β-oxidation. The customized PPARA stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Anthony

The ideal PPARA cell line, often used in our laboratories. Jun 16 2022

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chat William

The human PPARA overexpression cell line is a good product for general routine use from Creative Biolabs. Dec 11 2020

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FAQ

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Published Data

Fig.1 PPARα is down-regulated in human HCCs and cell lines.

In human HCC cell lines, PPARα mRNA expression is either down-regulated or silenced, whereas it is freely expressed in samples of normal liver tissue.

Ref: Zhang, Ning, et al. "Peroxisome proliferator activated receptor alpha inhibits hepatocarcinogenesis through mediating NF-κB signaling pathway." Oncotarget 5.18 (2014): 8330.

Pubmed: 25327562

DOI: 10.18632/oncotarget.2212

Research Highlights

Due to its aggressive nature and lack of effective treatment options, hepatocellular carcinoma (HCC) is the second most common cause of cancer-related mortality. Liver cancer is among the many tumors whose pathophysiology has been linked to microRNAs.
Drakaki, Alexandra, et al. "Functional microRNA high throughput screening reveals miR-9 as a central regulator of liver oncogenesis by affecting the PPARA-CDH1 pathway." BMC cancer 15.1 (2015): 1-11.
Pubmed: 26206264 DOI: 10.1186/s12885-015-1562-9

To reevaluate the significance of the peroxisome proliferator activated receptor alpha (PPARA) gene intron 7 G/C polymorphism (rs4253778) in athletes' high ability on endurance sports, a meta-analysis was conducted.
Lopez-Leon, Sandra, Catherine Tuvblad, and D. A. Forero. "Sports genetics: the PPARA gene and athletes' high ability in endurance sports. A systematic review and meta-analysis." Biology of sport 33.1 (2016): 3-6.
Pubmed: 26985127 DOI: 10.5604/20831862.1180170