mProX™ Human PKN1 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- Kinase Cell Lines
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Patrick Liam (Creative Biolabs Scientific Support)
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Patrick Liam (Creative Biolabs Scientific Support)
Published Data
Fig.1 Impact of PKN1 knockdown on GBM cells' biological behaviors.
In the colony formation assay, cells from both the A172 and U87 lines were transfected with siR-PKN1, and the resulting formations were observed, with a scale bar of 6 mm employed. It was observed that in comparison to the siR-NC groups, a significant reduction in proliferation viability was noted within the siR-PKN1 groups.
Ref: Hao, Yubing, et al. "The role of PKN1 in glioma pathogenesis and the antiglioma effect of raloxifene targeting PKN1." Journal of Cellular and Molecular Medicine 27.18 (2023): 2730-2743.
Pubmed: 37480215
DOI: 10.1111/jcmm.17860
Research Highlights
S Malik, Haleema. et al. "Phosphoprotein phosphatase activity positively regulates oligomeric pyrin to trigger inflammasome assembly in phagocytes." mBio, 2023.
In this study, the role of pyrin, a pattern-recognition receptor in phagocytes, was examined in relation to caspase-1 inflammasome assembly, triggered by bacterial toxins and the inactivation of RhoA. Pyrin, subject to negative regulation through phosphorylation by PKN1/2 kinases, plays a key role in Familial Mediterranean Fever (FMF) due to gain of function variants with a lower threshold for inflammasome assembly. The study focused on the identification of a phosphoprotein phosphatase (PPP) that positively regulates pyrin, using experiments with murine macrophages and FMF patient's monocytes. Results indicated that PPP activity is essential for dephosphorylating pyrin, with PP2A implicated in the dephosphorylation of S205 and the positive regulation of pyrin following RhoA inactivation.
S Malik, Haleema. et al. "Phosphoprotein phosphatase activity positively regulates oligomeric pyrin to trigger inflammasome assembly in phagocytes." mBio, 2023.
Pubmed:
37787552
DOI:
10.1128/mbio.02066-23
Sangiorgi, Eugenio. et al. "Identification by Exome Sequencing of Predisposing Variants in Familial Cases of Autoinflammatory Recurrent Fevers." Genes, 2023.
Periodic fever syndromes are characterized by recurring fevers and multi-organ inflammation without the involvement of T or B cells or autoantibodies. While the genetic architecture of many of these disorders remains complex, some, such as familial Mediterranean fever, have a classical monogenic origin and Mendelian transmission. Although gene panels are available for molecular diagnosis, they only have a diagnostic yield of 15-25%. This may be due to elusive causative genes, either through recessive or dominant variants, or the cumulative impact of multiple variants in more than one gene. In this study, exome sequencing was used to identify potential monogenic causes in five familial cases of autosomal dominant AID. Variants were identified through prioritization, filtering, and segregation analysis, and further evaluated using bioinformatics and pathway analysis to identify the most likely candidate genes for each family.
Sangiorgi, Eugenio. et al. "Identification by Exome Sequencing of Predisposing Variants in Familial Cases of Autoinflammatory Recurrent Fevers." Genes, 2023.
Pubmed:
37510214
DOI:
10.3390/genes14071310
