mProX™ Human PIM1 Stable Cell Line

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;ACC-MESO-1;CRL-5915

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research

Related Diseases

Primary Central Nervous System Lymphoma; Polyploidy

Gene ID

Human:5292

UniProt ID

Human:P11309

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

PIM1 has various applications in different areas. In liver cancer, PIM1 is involved in the growth of liver cancer cells and affects the transcriptional and translational abilities of multiple genes, as well as signaling pathways related to liver cancer. In Saccharomyces cerevisiae, PIM1 is essential for maintaining low levels of the mitochondrial HMG-box protein Cim1, which negatively regulates mtDNA copy number. In lower extremity deep venous thrombosis, PIM1 is part of the PIM1/FOXO3a axis and is involved in regulating apoptosis and angiogenesis. In the context of pathogen infection, PIM1 interacts with GBP1 to limit self-damage and protect against pathogen infection. Lastly, in colorectal cancer, PIM1 enhances the radiosensitivity of BRAF-mutated CRC cells by affecting the cell cycle, DNA damage, and tumor apoptosis. Overall, PIM1 plays a role in cancer growth, mitochondrial function, thrombosis, immune response, and radiotherapy sensitivity.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Jordan Smith (Verified Customer)

How does PIM1 mutation affect diffuse large B-cell lymphoma? Apr 08 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

PIM1 genetic alterations in diffuse large B-cell lymphoma are associated with distinct molecular profiles, phenotypes, and drug responses, highlighting its role as a personalized therapeutic target. Apr 08 2022

chat Skyler Davis (Verified Customer)

Can natural compounds inhibit PIM1 kinase for cancer treatment? Jan 05 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

Certain phytoconstituents have been identified as potent inhibitors of PIM1 kinase, suggesting their potential in anticancer drug discovery. Jan 05 2022

Published Data

Fig.1 Knockdown of PIM1 in mesothelioma cell lines.

A western blot analysis was conducted to evaluate PIM1 expression in ACC-MESO-1 and CRL-5915 cells following transfection with PIM-siRNA or NC-siRNA for a period of 2 days.

Ref: Mawas, Amany Sayed, et al. "PIM1 knockdown inhibits cell proliferation and invasion of mesothelioma cells." International Journal of Oncology 50.3 (2017): 1029-1034.

Pubmed: 28197633

DOI: 10.3892/ijo.2017.3863

Research Highlights

Song, Shuting. et al. "miR-3200 accelerates the growth of liver cancer cells by enhancing Rab7A." Non-coding RNA research, 2023.
Recent studies have shown a close association between miR-3200 and tumorigenesis. However, the exact role of miR-3200 in human hepatocarcinogenesis remains unclear. This current research aims to elucidate the impact of miR-3200 on the progression of liver cancer cells. The findings of this study provide clear evidence that miR-3200 promotes the growth of liver cancer cells, shedding light on its significance in the development of this disease. These results have implications for potential therapeutic targets for hepatocellular carcinoma.
Song, Shuting. et al. "miR-3200 accelerates the growth of liver cancer cells by enhancing Rab7A." Non-coding RNA research, 2023.
Pubmed: 37860266 DOI: 10.1016/j.ncrna.2023.10.005

Schrott, Simon; Osm, Christof. "Two mitochondrial HMG-box proteins, Cim1 and Abf2, antagonistically regulate mtDNA copy number in Saccharomyces cerevisiae." Nucleic acids research, 2023.
In this study, a previously unknown mitochondrial HMG-box protein, Cim1, was discovered in Saccharomyces cerevisiae. It was found that Cim1's association with mtDNA depends on the cell's metabolic state. Unlike Abf2, which serves as a supportive factor in mtDNA maintenance, Cim1 plays a negative role in controlling mtDNA copy number. Deletion of Cim1 increases mtDNA levels and enhances mitochondrial function, while its overexpression leads to mtDNA loss. Notably, the absence of Cim1 alleviates mtDNA maintenance issues caused by Abf2 loss, and Cim1 overexpression effects can be mitigated by concurrent Abf2 overexpression. Additionally, the enzyme Pim1 is crucial in maintaining low Cim1 levels, preventing its accumulation and repressive effects on mtDNA. This study proposes a model where the balance between antagonistic Cim1 and Abf2 proteins regulates mtDNA copy number.
Schrott, Simon; Osm, Christof. "Two mitochondrial HMG-box proteins, Cim1 and Abf2, antagonistically regulate mtDNA copy number in Saccharomyces cerevisiae." Nucleic acids research, 2023.
Pubmed: 37850632 DOI: 10.1093/nar/gkad849