mProX™ Human PGR Stable Cell Line

Product Information

Target Protein

PGR

Target Family

3-Ketosteroid Receptor

Target Protein Species

Human

Host Cell Type

NCI-H295R; MUC-1; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Cancer Research; Reproductive Research

Related Diseases

Progesterone Resistance; Myoma

Gene ID

5241

UniProt ID

P06401

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The progesterone receptor (PR), which is a protein present inside cells, is sometimes referred to as nuclear receptor subfamily 3, group C, member 3 (NR3C3). Progesterone is a steroid hormone that activates it.
To cause the progesterone receptors to become activated, progesterone is required. Transcription is inhibited by the carboxyl terminus in the absence of binding hormone. The inhibitory effect is eliminated by a structural alteration brought about by hormone binding. Antagonisms to progesterone block the structural reorganization. Progesterone attaches itself to the receptor, and then the complex reaches the nucleus and binds to DNA by restructuring and dimerization. There, transcription occurs and messenger RNA is formed. Ribosomes then translate the messenger RNA to make target proteins. The customized PGR stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat David

The PGR cell line was an indispensable tool in my research. The cell line's stability and reproducibility were outstanding, allowing for reliable results across multiple experiments. Nov 17 2022

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chat William

The PGR cell line has played a pivotal role in our journey of discovery. Its reliable performance and consistent results have propelled our research forward. Mar 09 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 PgR expression in NCI-H295R, MUC-1 cell lines and ACC115m primary culture.

On coverslips that had been pre-treated with poly-L-lysine, cells were sown, and then DAPI was added for nuclear staining. Panels A, B, C, and D are DAPI, phalloidin, PgR, and merge, respectively.

Ref: Rossini, Elisa, et al. "Cytotoxic effect of progesterone, tamoxifen and their combination in experimental cell models of human adrenocortical cancer." Frontiers in Endocrinology 12 (2021): 669426.

Pubmed: 33981288

DOI: 10.3389/fendo.2021.669426

Research Highlights

For various legumes and non-legumes, PGPR can serve as a biofertilizer and bioenhancer. The application of PGPR and symbiotic microbes may be helpful in the creation of plans to support plants' water saving. It has been established that applying PGPR to increase crop yields in an environmentally responsible manner is possible by either directly or indirectly promoting plant development.
Khan, Naeem, et al. "Interaction between PGPR and PGR for water conservation and plant growth attributes under drought condition." Biologia 73 (2018): 1083-1098.

The Pgr-KO study provides the first strong evidence that Pgr is not necessary for starting non-genomic progestin signaling and inducing meiosis resumption, as well as supporting other findings that show a role for Pgr in steroid-dependent genomic signaling pathways leading to ovulation.
Zhu, Yong, et al. "Nuclear progestin receptor (pgr) knockouts in zebrafish demonstrate role for pgr in ovulation but not in rapid non-genomic steroid mediated meiosis resumption." Frontiers in endocrinology 6 (2015): 37.
Pubmed: 25852646 DOI: 10.3389/fendo.2015.00037