mProX™ Human PDGFRB Stable Cell Line

Product Information

Target Protein

PDGFRB

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

Mero-14; IstMes2; Mero-25; Met5A; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research; Cardiovascular Research; Digestive and Renal Research

Related Diseases

Premature Aging Syndrome; Penttinen Type and Kosaki Overgrowth Syndrome

Gene ID

5159

UniProt ID

P09619

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The gene PDGFRB codes for the production of a protein known as platelet-derived growth factor receptor beta (PDGFRβ), a member of the receptor tyrosine kinase protein family. Certain cell types include the PDGFRβ protein in their cell membrane, where it is attached to a protein known as platelet-derived growth factor. The PDGFRβ protein is activated by this interaction, and it adds a cluster of oxygen and phosphorus atoms at precise locations to other proteins within the cell to make them active. PDGFRβ protein-stimulated signaling pathways govern numerous vital cellular functions, including growth and division, motility, and survival. The signaling of the PDGFRβ protein is essential for the growth of several cell types in the body. The customized PDGFRB stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Shirley

I think this human PDGFRB cell line is better than many similar products already on the market. Aug 22 2020

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chat Rebecca

This is a very reliable mouse PDGFRB cell line with high sensitivity and specificity, suitable for experiments such as immunofluorescence microscopy and flow cytometry. Sep 11 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Expression levels of PDGFRB in Mero-14, IstMes2, and Mero-25 human MPM cell lines and Met5A.

Analyses of protein expression produced comparable findings. Mero-14 cells exhibited the highest levels of PDGFRB protein, with an average increase of 99% in comparison to Met5A cells. The IstMes2 cell line experienced a 70% increase, whereas Mero-25 cells displayed a 97% increase.

Ref: Melaiu, Ombretta, et al. "Inhibition of the platelet-derived growth factor receptor beta (PDGFRB) using gene silencing, crenolanib besylate, or imatinib mesylate hampers the malignant phenotype of mesothelioma cell lines." Genes & cancer 8.1-2 (2017): 438.

Pubmed: 28435517

DOI: 10.18632/genesandcancer.129

Research Highlights

Early detection of these tumors is critical because, in contrast to patients with tumors associated with FGFR1 fusions, those with tumors associated with PDGFRA or PDGFRB fusions frequently respond to tyrosine kinase inhibitor therapy.
Vega, Francisco, et al. "Hematolymphoid neoplasms associated with rearrangements of PDGFRA, PDGFRB, and FGFR1." American journal of clinical pathology 144.3 (2015): 377-392.
Pubmed: 26276769 DOI: 10.1309/AJCPMORR5Z2IKCEM

Most recently, heterozygous mutations in the PDGFB and PDGFRB genes have been connected to Primary Familial Brain Calcification (PFBC), a neurological disease characterized by increasing pericapillary calcifications.
Vanlandewijck, Michael, et al. "Functional characterization of germline mutations in PDGFB and PDGFRB in primary familial brain calcification." PloS one 10.11 (2015): e0143407.
Pubmed: 26599395 DOI: 10.1371/journal.pone.0143407