mProX™ Human PDGFRA Stable Cell Line

Product Information

Target Protein

PDGFRA

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research; Cardiovascular Research; Digestive and Renal Research

Related Diseases

Gist-Plus Syndrome; Hypereosinophilic Syndrome

Gene ID

5156

UniProt ID

P16234

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The PDGFRA gene codes for the production of the protein platelet-derived growth factor receptor alpha (PDGFRA), a member of the receptor tyrosine kinase (RTK) family of proteins. A process known as signal transduction is used by receptor tyrosine kinases to transfer messages from the cell surface within the cell. In the membrane of some cell types, there is a protein known as platelet-derived growth factor that binds to the PDGFRA protein. The PDGFRA protein is activated by this interaction, and it adds a cluster of oxygen and phosphorus atoms at precise locations to other proteins within the cell to make them active. Numerous signaling pathways see a number of proteins activated as a result of this activity. The customized PDGFRA stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Sarah

Most importantly, I would recommend this product to anyone who wants to research PDGFRA. Aug 09 2020

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chat Linda

This PDGFRA KO cell line has saved us time and resources. Our team is excited to delve deeper into our investigations with this versatile tool. Feb 23 2021

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 PDGFRA surface expression is controlled by cytoskeleton system.

Pharmacological inhibitors were used to impair the cytoskeletal organization of glioma cell lines #1, #2, and #3 that had high surface PDGFRA expression in order to evaluate the effects of the cytoskeleton on surface PDGFRA expression in living glioma cells.

Ref: Chen, Dongfeng, et al. "Glioma cell proliferation controlled by ERK activity-dependent surface expression of PDGFRA." PLoS One 9.1 (2014): e87281.

Pubmed: 24489888

DOI: 10.1371/journal.pone.0087281

Research Highlights

KIT or PDGFRA receptor tyrosine kinases are the sites of activating mutations found in most gastrointestinal stromal tumors (GIST). The KIT/PDGFRA tyrosine kinase inhibitor (TKI) imatinib's approval significantly enhanced the management of advanced GIST.
Bauer, Sebastian, et al. "Early and next-generation KIT/PDGFRA kinase inhibitors and the future of treatment for advanced gastrointestinal stromal tumor." Frontiers in Oncology 11 (2021): 672500.
Pubmed: 34322383 DOI: 10.3389/fonc.2021.672500

Among GISTs, PDGFRA IHC can be positive for inflammatory fibroid polyps and some other mesenchymal tumor types. It is also quite sensitive and somewhat selective for PDGFRA-mutant tumors. To identify the best course of treatment, epithelioid GISTs with PDGFRA positive could be categorized for PDGFRA sequencing.
Papke Jr, David J., et al. "PDGFRA immunohistochemistry predicts PDGFRA mutations in gastrointestinal stromal tumors." The American Journal of Surgical Pathology 46.1 (2022): 3-10.
Pubmed: 33859072 DOI: 10.1097/PAS.0000000000001720