mProX™ Human PAK3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX417	Magic™ Human PAK3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;HepG2;Huh-7;Hep3B

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Intellectual Developmental Disorder, X-Linked 30; Non-Syndromic X-Linked Intellectual Disability

Gene ID

Human:8874

UniProt ID

Human:Q14155

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

PAK3 (p21-activated kinase 3) has various applications in different areas. In one study, a splicing variant of the PAK3 gene was found to be associated with X-linked intellectual disability in a child, expanding the understanding of genetic causes and providing a basis for genetic counseling and prenatal diagnosis. Another study showed that EZH2, a neuronal histone methyltransferase, regulates genes related to neuronal morphogenesis, including the gene encoding PAK3, which is important for dendritic spine density and cognitive behavior. Additionally, PAK3 was found to be involved in cardiac dysfunction, with its overexpression leading to pathological remodeling and deteriorated heart function. Targeting PAK3 has potential therapeutic implications for metastatic prostate cancer by addressing cell survival, proliferation, and motility. Lastly, HBV DNA integration into the human genome was shown to upregulate PAK3 expression, suggesting its involvement in hepatocarcinogenesis. These findings highlight the diverse roles of PAK3 in intellectual disabilities, neuronal morphogenesis, cardiac dysfunction, cancer, and hepatocellular carcinoma.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Casey Smith (Verified Customer)

How does PAK3 contribute to the metastasis of hepatocellular carcinoma? Jul 23 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

PAK3 enhances the invasion of hepatocellular carcinoma (HCC) by regulating the epithelial-mesenchymal transition (EMT) process, suggesting its potential as a treatment target. Jul 23 2023

chat Casey Johnson (Verified Customer)

What is the impact of PAK3 on stress-induced cardiac hypertrophy? Sep 09 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

Overexpression of PAK3 in stress-induced cardiac hypertrophy dysregulates autophagy, leading to acute cell death and accelerated systolic dysfunction. Sep 09 2020

Published Data

Fig.1 PAK3 regulates the TGFβ/Smad pathway.

Increased Smad2 and Smad3 phosphorylation is observed with PAK3 overexpression, while decreased phosphorylation is observed with PAK3 knockdown, without significant impact on total Smad2 or Smad3 expression, as demonstrated by Western blotting assays.

Ref: Gao, Zhi, et al. "PAK3 promotes the metastasis of hepatocellular carcinoma by regulating EMT process." Journal of Cancer 13.1 (2022): 153.

Pubmed: 34976179

DOI: 10.7150/jca.61918

Research Highlights

Wang, Chen. et al. "Analysis of a child with X-linked intellectual disability due to a maternal de novo splicing variant of the PAK3 gene." Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics, 2023.
The aim of the study was to investigate the genetic causes of profound intellectual disabilities and prominent behavioral abnormalities in a child. The researchers aimed to identify potential genetic factors contributing to the child's condition using various genetic analysis methods. This study has implications for understanding the genetic etiology of intellectual disabilities and may aid in the development of targeted treatments for individuals with similar symptoms. The findings support the importance of considering genetic factors in the diagnosis and management of intellectual disabilities.
Wang, Chen. et al. "Analysis of a child with X-linked intellectual disability due to a maternal de novo splicing variant of the PAK3 gene." Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics, 2023.
Pubmed: 37368392 DOI: 10.3760/cma.j.cn5113874-20220513-00326

Zhang, Mei. et al. "Neuronal Histone Methyltransferase EZH2 Regulates Neuronal Morphogenesis, Synaptic Plasticity, and Cognitive Behavior in Mice." Neuroscience bulletin, 2023.
The role of the histone methyltransferase enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2)-mediated trimethylation of histone H3 lysine 27 (H3K27me3) in regulating neural stem cell proliferation and fate specificity in the central nervous system has been studied. A neuron-specific Ezh2 conditional knockout mouse line was used to investigate the function of EZH2 in early post-mitotic neurons. Results showed that a lack of neuronal EZH2 resulted in delayed neuronal migration, increased dendritic complexity, and elevated dendritic spine density. Transcriptome analysis revealed neuronal EZH2-regulated genes related to neuronal morphogenesis, with the gene encoding p21-activated kinase 3 (Pak3) identified as a target gene suppressed by EZH2 and H3K27me3. The expression of dominant negative Pak3 was able to reverse the higher dendritic spine density caused by Ezh2 knockout. Additionally, adult mice lacking neuronal EZH2 exhibited impaired memory behaviors, suggesting long-lasting effects on cognitive function. These findings demonstrate the role of neuronal EZH2 in regulating multiple aspects of neuronal morphogenesis during development, with ongoing effects on cognitive function in adulthood.
Zhang, Mei. et al. "Neuronal Histone Methyltransferase EZH2 Regulates Neuronal Morphogenesis, Synaptic Plasticity, and Cognitive Behavior in Mice." Neuroscience bulletin, 2023.
Pubmed: 37326884 DOI: 10.1007/s12264-023-01074-1