mProX™ Human P2RY8 Stable Cell Line

Product Information

Target Protein

P2RY8

Target Family

P2Y Family

Target Protein Species

Human

Host Cell Type

CRLF2; Ba/F3; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cancer Research; Cardiovascular Research

Related Diseases

B-Cell Acute Lymphoblastic Leukemia

Gene ID

286530

UniProt ID

Q86VZ1

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

P2RY8 is a transmembrane receptor that binds to and is activated most selectively by adenosine and uridine nucleotides. Adenosine and uridine nucleotides stimulate P2RY8 activity, which in turn stimulates the intracellular signaling pathway for adenylyl cyclase. P2RY8 can activate phospholipase C and the extracellular signal-regulated kinases signaling pathway after binding to nucleotides. There are also several studies that demonstrate the role of purinergic receptor signaling in a number of biological processes, including cell migration, proliferation, differentiation, and apoptosis. P2RY8 may play a role in leukemogenesis as patients with high levels of P2RY8 expression have a higher chance of developing leukemogenesis than individuals with low levels of P2RY8 expression. The customized P2RY8 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Abigail

mProX™ Human P2RY8 Stable Cell Line is very good! Jun 06 2023

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chat Matthew

The P2RY8 cell line is of superior quality, and I am confident in its reliability. Aug 20 2023

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FAQ

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Published Data

Fig.1 P2RY8-CRLF2 and HMGN1 cooperate for cytokine-independent growth.

Using flow cytometry to quantify the surface expression of TSLPR-APC in Ba/F3 HMGN1 and CRLF2 cell lines deprived of IL-3 for 6 hours.

Ref: Page, Elyse C., et al. "HMGN1 plays a significant role in CRLF2 driven Down Syndrome leukemia and provides a potential therapeutic target in this high-risk cohort." Oncogene 41.6 (2022): 797-808.

Pubmed: 34857887

DOI: 10.1038/s41388-021-02126-4

Research Highlights

A major source of B cell malignancies, germinal centers are significant locations for antibody diversification and affinity development. Uncertainty surrounds the cues that encourage this restriction. A Gα13-coupled receptor called P2RY8 controls the expansion of B cells in lymphoid organs and mediates their restriction of migration.
Lu, Erick, et al. "S-Geranylgeranyl-L-glutathione is a ligand for human B cell-confinement receptor P2RY8." Nature 567.7747 (2019): 244-248.
Pubmed: 30842656 DOI: 10.1038/s41586-019-1003-z

P2RY8 overexpression in cells and animals inhibited the growth of plasma cells and strengthened the negative selection of developing B cells with DNA reactivity. These findings reveal a function for P2RY8 in the development of lupus and immunological tolerance.
He, Yuke, et al. "P2RY8 variants in lupus patients uncover a role for the receptor in immunological tolerance." Journal of Experimental Medicine 219.1 (2021): e20211004.
Pubmed: 34889940 DOI: 10.1084/jem.20211004