mProX™ Human P2RY11 Stable Cell Line

Product Information

Target Protein

P2RY11

Target Family

P2Y Family

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cancer Research; CNS Research

Related Diseases

Narcolepsy

Gene ID

5032

UniProt ID

Q96G91

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

A member of the G-protein coupled receptors (GPCR) family, P2RY11 is a 40.3 kDa protein. The human P2RY11 is 33% identical to the human P2RY1 (its closest homolog), and it is activated by adenine but not by the uridine nucleotide, which speeds up the breakdown of phosphoinositides that are resistant to pertussis toxin as well as the production of cyclic AMP. This receptor functions as a selective purinoceptor and is connected to G protein, which promotes the phosphoinositide and adenylyl cyclase pathways. It has been discovered that naturally occurring read-through transcripts, produced by the intergenic splicing of this gene and an upstream gene called PPAN, exist. Everywhere you look, you can find the PPAN-P2RY11 read-through mRNA, which produces a fusion protein that is identical to each gene's product. Additionally, there is a connection between P2RY11 and a number of disorders, such as sleep disorder narcolepsy. The customized P2RY11 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The P2RY11 cell line is an essential tool for anyone studying drug discovery. Jun 17 2023

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The P2RY11 cell line is very good to use and is of high quality and safety. May 27 2023

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FAQ

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Published Data

Fig.1 P2RY11 mRNA expression in PBMCs.

Both CD8+ T cells and natural killer cells showed the effects of the disease-associated allele on gene expression, but not other PBMC subtypes. The major influence on CD8+ T cells and natural killer cells, which make up about 25% of all PBMCs, is consistent with the smaller genotype effect on expression in PBMCs.

Ref: Kornum, Birgitte R., et al. "Common variants in P2RY11 are associated with narcolepsy." Nature genetics 43.1 (2011): 66-71.

Pubmed: 21170044

DOI: 10.1038/ng.734

Research Highlights

A very specific loss of hypocretin (orexin) neurons is a hallmark of the sleep disorder narcolepsy with cataplexy, which has led to the theory that the disorder is brought on by an immunological or autoimmune mechanism. All narcolepsy-related genetic variations have immune-related roots. Single nucleotide variations at the P2RY11-EIF3G gene are among them.
Degn, Matilda, et al. "Rare missense mutations in P2RY11 in narcolepsy with cataplexy." Brain 140.6 (2017): 1657-1668.
Pubmed: 28460015 DOI: 10.1093/brain/awx093

The family of purinergic receptors includes the P2Y11 receptor. It has been underappreciated in part because the murine genome lacks an ortholog of the P2ry11 gene, which hinders the creation of knockout mice, which have been crucial for identifying the functions of other P2Y receptors.
Dreisig, Karin, and Birgitte Rahbek Kornum. "A critical look at the function of the P2Y 11 receptor." Purinergic Signalling 12 (2016): 427-437.
Pubmed: 27246167 DOI: 10.1007/s11302-016-9514-7