mProX™ Human OPRL1 Stable Cell Line

Xiaoqin Z, et al. "Development of a prognostic signature for immune-associated genes in bladder ." International urology and nephrology, 2023.
Bladder cancer predominantly affects men and is a common malignancy of the urinary system. Although surgery alone is typically the main treatment option, platinum-based chemotherapy has shown some improvements in overall survival. However, conventional chemotherapy can come with unpleasant side effects, prompting researchers to explore alternative therapies such as immunotherapy. In this study, the authors utilized bioinformatics analyses on The Cancer Genome Atlas (TCGA) database to develop an immune-associated prognostic signature for bladder cancer. This signature included 1 LncRNA (NR2F1-AS1) and 16 mRNAs, which were used to predict a potential therapeutic drug through the Connectivity Map database. The drug, Cephaeline, was found to effectively inhibit the growth of T24 bladder cancer cells. Ultimately, this new predictive pattern enables more targeted and effective treatment for bladder cancer patients and lays the groundwork for future immunotherapy trials in this field.
Pubmed: 37740848   DOI: 10.1007/s11255-023-03796-7

Hu R, et al. "Selection signature analysis reveals RDH5 performed key function in vision during ." Archives animal breeding, 2023.
In the Neolithic age, sheep were one of the most successful domesticated animals and gradually spread across the world through human activities. The domestication process resulted in changes in the sheep's morphology, physiology, and behavior, leading to the emergence of different breeds with unique characteristics through artificial and natural selection. However, the underlying genetic basis responsible for these variations remains largely unidentified. This study utilized whole genome resequencing technology to compare and analyze the genome differences between Asiatic mouflon wild sheep (Ovis orientalis) and Hu sheep (Ovis aries). Through this analysis, 755 genes were identified to have undergone positive selection during the domestication and selection process. Of interest were the genes related to sensory perception that displayed directional evolution in the autosomal region, including OPRL1, LEF1, TAS1R3, ATF6, VSX2, MYO1A, RDH5, and several novel genes. A missense mutation, c.T722C/p.M241T, was discovered in exon 4 of RDH5 in sheep, and the T allele was completely fixed in Hu sheep. Furthermore, the mutation with the C allele resulted in reduced retinol dehydrogenase activity encoded by RDH5, affecting retinoic acid metabolism and influencing the visual cycle. In conclusion, this study demonstrated that during sheep domestication, there was a significant enrichment for positively selected genes involved in sensory perception development. Additionally, the missense mutation in RDH5 and its variants may be associated with retinal degeneration in sheep. It is hypothesized that humans eliminated wild sheep ancestors with weaker visual sensitivity, and the mutation was selectively favored under the dual pressures of natural and artificial selection.
Pubmed: 37384328   DOI: 10.5194/aab-66-81-2023