mProX™ Human OPN5 Stable Cell Line

Sato, Keita; Ohuc, Hideyo. "Molecular property, manipulation, and potential use of Opn5 and its homologs." Journal of molecular biology, 2023.
The G-protein coupled receptor known as animal opsin is a vital component in forming photopigments by binding with retinal as a chromophore. The Opsin 5 (Opn5) group, a subset of the animal opsin family, contains a variety of related proteins, including Opn5m found in all vertebrates, Opn5L1 and Opn5L2 in non-mammalian vertebrates, and Opn6 in non-therian vertebrates, as well as Opn5 homologs in invertebrates. Despite belonging to the same clade in the molecular phylogenetic tree, these proteins have distinct characteristics in areas such as retinal binding, photoreaction, and G-protein coupling. While their role in physiological functions is acknowledged, more research is needed to fully understand their functions and unique molecular properties. In particular, these properties suggest that Opn5 proteins have potential as optogenetic tools and can be manipulated for specific purposes. This paper provides an overview of the current knowledge on Opn5, explores potential manipulation approaches for its molecular characteristics, and discusses its potential applications in the rapidly growing field of animal opsin optogenetics.
Sato, Keita; Ohuc, Hideyo. "Molecular property, manipulation, and potential use of Opn5 and its homologs." Journal of molecular biology, 2023.
Pubmed: 37865286   DOI: 10.1016/j.jmb.2023.168319

Jeong, Heonuk. et al. "Opsin 5 mediates violet light-induced early growth response-1 expression in the mouse retina." Scientific reports, 2023.
Myopia, an abnormal vision condition that causes difficulties in seeing distant objects, has become a growing concern in both Asian and Western countries. Previous research has shown that exposure to violet light (VL, 360-400 nm wavelength) can effectively suppress myopia progression in experimental chick and mice models. However, the role of Opn5, a specific protein found in the retina, in this process is still unknown. The aim of this study was to investigate whether the upregulation of early growth response-1 (Egr-1) expression, another protein involved in myopia, induced by VL exposure is dependent on Opn5 expression in the mouse retina. The results showed that both Egr-1 mRNA and protein expressions were significantly increased in the mouse retina and mouse retinal 661W cells following VL exposure. However, this increase was reduced in both retina-specific Opn5 conditional knockout (KO) mice and Opn5 KO 661W cells, suggesting that Opn5 mediates VL-induced EGR-1 upregulation in mice. These findings could potentially lead to the development of new molecular targets for the prevention and treatment of myopia.
Jeong, Heonuk. et al. "Opsin 5 mediates violet light-induced early growth response-1 expression in the mouse retina." Scientific reports, 2023.
Pubmed: 37857760   DOI: 10.1038/s41598-023-44983-x