mProX™ Human NTSR1 Stable Cell Line

Chen YL, et al. "LncRNA SLCO4A1-AS1 suppresses lung cancer progression by sequestering the ." Journal of biomedical science, 2023.
Metastasis is a complex process that involves the spread of cancer cells to other parts of the body and is a major characteristic of malignant tumors. Recent research has revealed that long non-coding RNAs (lncRNAs) play a crucial role in regulating metastasis. This study aims to examine the involvement of a specific lncRNA, solute carrier organic anion transporter family member 4A1-antisense 1 (SLCO4A1-AS1), in metastasis and its underlying mechanisms. Through comprehensive analysis of the Gene Expression Omnibus (GEO) database, the researchers identified SLCO4A1-AS1 as a potential metastasis-associated lncRNA. In vitro and in vivo experiments were then conducted to assess the impact of SLCO4A1-AS1 on cancer cell migration and invasion. High-throughput screening methods, including MASS Spectrometry and RNA sequencing, were used to identify the downstream targets of SLCO4A1-AS1. Further experiments, such as RT-qPCR, western blotting, RNA pull-down, RNA immunoprecipitation, fluorescence in situ hybridization, and chromatin immunoprecipitation, were conducted to validate the regulatory mechanisms of SLCO4A1-AS1. Results showed that SLCO4A1-AS1 can inhibit cancer cell migration and invasion by disrupting the cytoskeleton filaments. Additionally, it was associated with longer overall survival in patients with lung adenocarcinoma. Further investigation revealed that SLCO4A1-AS1 directly interacts with a DNA-binding protein called TOX High Mobility Group Box Family Member 4 (TOX4), inhibiting its ability to promote migration and invasion. Furthermore, RNA sequencing uncovered that neurotensin receptor 1 (NTSR1) is a downstream target of both SLCO4A1-AS1 and TOX4. It was found that SLCO4A1-AS1 acts as a decoy of TOX4, preventing its interaction with the NTSR1 promoter and thus preventing NTSR1 transcription. In terms of function, NTSR1 has been shown to promote cancer cell migration and invasion through cytoskeletal remodeling, and knocking down NTSR1 was found to significantly inhibit TOX4-induced migration and invasion. The study concludes that SLCO4A1-AS1 plays a key role in suppressing TOX4/NTSR1 signaling,
Pubmed: 37726723   DOI: 10.1186/s12929-023-00973-9

Lee Y, et al. "Mechanisms underlying probiotic effects on neurotransmission and stress ." Fish & shellfish immunology, 2023.
In a study conducted by the authors, a 1-month lab-scale trial and a 6-month field-scale trial were conducted to investigate the effects of probiotic administration on fish health. The probiotic strain Lactococcus lactis WFLU12 was used in both trials. Liver samples were collected from the fish at one-month post-feeding (mpf) in both trials and analyzed using RNA-Seq. Fish growth was monitored monthly and serological parameters were measured at one mpf in the field-scale experiment. The results of the lab-scale trial showed that the administration of probiotics significantly upregulated genes related to neurotransmission, including htr3a, mao, ddc, ntsr1, and gfra2, demonstrating the impact of probiotics on the microbiota-gut-brain axis. In the field-scale experiment, fish growth was significantly improved and the levels of AST, LDH, and cortisol in the sera were higher in the control group compared to the probiotics group. In addition, genes involved in stress responses (e.g. hsp70, hsp90B1, hspE1, prdx1, and gss) and transcriptional regulators (e.g. fos, dusp1, and dusp2) were significantly upregulated in the control group, indicating that probiotic administration can alleviate stress levels in fish. Overall, this study provides valuable insights into the mechanisms by which probiotics can positively impact fish health, particularly in regards to neurotransmission and stress alleviation.
Pubmed: 37678478   DOI: 10.1016/j.fsi.2023.109063