mProX™ Human NR3C2 Stable Cell Line

Product Information

Target Protein

NR3C2

Target Family

3-Ketosteroid Receptor

Target Protein Species

Human

Host Cell Type

Panc-1; MiaPaCa-2; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Inflammation Research; Reproductive Research

Related Diseases

Pseudohypoaldosteronism

Gene ID

4306

UniProt ID

P08235

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Instructions for producing the protein known as the mineralocorticoid receptor are found in the NR3C2 gene. Specialized proteins in the cell membrane that govern the entry of potassium or sodium into cells are regulated by the mineralocorticoid receptor. The mineralocorticoid receptor, particularly in some kidney cells, increases the quantity and activity of these proteins at the cell membrane in response to signals that the body's sodium levels are low. While one of these proteins moves sodium into the cell, the other protein moves potassium into the cell and sodium out of the cell at the same time. These proteins aid in the removal of potassium from the body through secretion and the maintenance of sodium in the body through reabsorption. The customized NR3C2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Cynthia

This is a very reliable NR3C2 cell line with high sensitivity and specificity, suitable for experiments such as immunofluorescence microscopy and flow cytometry. Feb 12 2020

chat Verified Customer

chat Robert

Recommended for all labs based on my experience of using this NR3C2 cell line. Jan 05 2021

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 NR3C2 inhibits proliferation, colony formation, invasiveness and epithelial-tomesenchymal transition.

Overexpression of NR3C2 inhibits Panc-1 and MiaPaCa-2 cell growth. Comparing NR3C2-overexpressing cells to control cells, fewer colonies were formed.

Ref: Yang, Shouhui, et al. "A novel MIF signaling pathway drives the malignant character of pancreatic cancer by targeting NR3C2." Cancer research 76.13 (2016): 3838-3850.

Pubmed: 27197190

DOI: 10.1158/0008-5472.CAN-15-2841

Research Highlights

Despite the fact that HPA-axis reactivity has been frequently linked to cognitive functioning, it is unclear whether this relationship is positive or negative. Therefore, genetic factors that affect both cognitive functioning and HPA-axis reactivity may help explain the relationship between stress and cognition.
Plieger, Thomas, et al. "The role of genetic variation in the glucocorticoid receptor (NR3C1) and mineralocorticoid receptor (NR3C2) in the association between cortisol response and cognition under acute stress." Psychoneuroendocrinology 87 (2018): 173-180.
Pubmed: 29100174 DOI: 10.1016/j.psyneuen.2017.10.020

The GA haplotype in NR3C2 and the mutation rs2070951 were linked to a higher incidence of cCSC in this investigation. The pathophysiology of cCSC may involve the mineralocorticoid receptor, according to the findings of this genetic investigation.
van Dijk, Elon HC, et al. "Association of a haplotype in the NR3C2 gene, encoding the mineralocorticoid receptor, with chronic central serous chorioretinopathy." JAMA ophthalmology 135.5 (2017): 446-451.
Pubmed: 28334414 DOI: 10.1001/jamaophthalmol.2017.0245