mProX™ Human NR3C1 Stable Cell Line

Product Information

Target Protein

NR3C1

Target Family

3-Ketosteroid Receptor

Target Protein Species

Human

Host Cell Type

CCRF-CEM; 6T-CEM; NALM6; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Inflammation Research; Reproductive Research

Related Diseases

Glucocorticoid Resistance; Generalized and Post-Traumatic Stress Disorder

Gene ID

2908

UniProt ID

P04150

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Cortisol and other glucocorticoids bind to a receptor called the glucocorticoid receptor, or NR3C1. Nearly all body cells express the GR, which controls genes involved in immune response, metabolism, and development. The receptor gene has a wide range of effects in many body areas due to its multiple modes of expression. The major mechanism of action of glucocorticoids when they bind to GR is the control of gene transcription. The unbound receptor is found in the cell's cytoplasm. There are two possible outcomes for the receptor-glucocorticoid complex once the receptor is coupled to the glucocorticoid. Pro-inflammatory protein expression in the cytosol is repressed by the activated GR complex, whereas anti-inflammatory protein expression in the nucleus is upregulated. The customized NR3C1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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This NR3C1 cell line has saved us time and resources. Our team is excited to delve deeper into our investigations with this versatile tool. Jan 08 2023

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I think this NR3C1 cell line is better than many similar products already on the market. Jul 18 2021

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FAQ

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Published Data

Fig.1 Effects of deletion of NR3C1 in glucocorticoid-sensitive ALL cells.

CRISPR guide RNA vector transfection was used to knock down the NR3C1 gene in CCRF-CEM, 6T-CEM, and NALM6 ALL cells. Three positive single clones (CCRF-CEM-KO, 6T-CEM-KO, and NALM6-KO) were selected, and their identities were verified using β-actin or anti-NR3C1 antibody western blotting analysis.

Ref: Xiao, Haowen, et al. "Haploinsufficiency of NR3C1 drives glucocorticoid resistance in adult acute lymphoblastic leukemia cells by down-regulating the mitochondrial apoptosis axis, and is sensitive to Bcl-2 blockage." Cancer cell international 19 (2019): 1-13.

Pubmed: 31462891

DOI: 10.1186/s12935-019-0940-9

Research Highlights

The stress response system depends on the glucocorticoid receptor gene (NR3C1). Since NR3C1 cytosine methylation has been frequently linked to trauma and mental illnesses such major depressive disorder, anxiety, PTSD, and personality disorders, it is possible that methylation of NR3C1 contributes to stress-related psychopathology.
Watkeys, Oliver J., et al. "Glucocorticoid receptor gene (NR3C1) DNA methylation in association with trauma, psychopathology, transcript expression, or genotypic variation: A systematic review." Neuroscience & Biobehavioral Reviews 95 (2018): 85-122.
Pubmed: 30176278 DOI: 10.1016/j.neubiorev.2018.08.017

This paper examines how the glucocorticoid receptor gene (NR3C1) is regulated in humans during the first 1000 days of life, both under protective and unfavorable environmental settings.
Berretta, Erica, et al. "Glucocorticoid receptor gene (NR3C1) methylation during the first thousand days: Environmental exposures and developmental outcomes." Neuroscience & Biobehavioral Reviews 125 (2021): 493-502.
Pubmed: 33689802 DOI: 10.1016/j.neubiorev.2021.03.003