mProX™ Human NR1I3 Stable Cell Line

Product Information

Target Protein

NR1I3

Target Family

Vitamin D Receptor

Target Protein Species

Human

Host Cell Type

Hap1; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Diabetes Research; Metabolic Research

Related Diseases

Biliary Tract Disease; Secondary Hyperparathyroidism

Gene ID

9970

UniProt ID

Q14994

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human NR1I3 gene encodes a protein known as the constitutive androstane receptor (CAR). CAR is a sensor of endobiotic and xenobiotic chemicals. It belongs to the nuclear receptor superfamily, along with pregnane X receptor (PXR). Proteins involved in the metabolism and excretion of these compounds have their expression increased in response.As a result, CAR and PXR are crucial to the detoxification of alien compounds like narcotics. Despite being natural antagonists of the CAR, androstenol and various isomers of androstanol, known as androstanes, served as the inspiration for the receptor's nomenclature. Dehydroepiandrosterone, or DHEA, has been discovered more recently to be an endogenous CAR agonist. The customized NR1I3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat James

I have tested the NR1I3 overexpression cell line from Creative Biolabs as one of the most reliable and versatile options available. Jul 01 2023

chat Verified Customer

chat Barbara

The NR1I3 cell line is easy to use, and the provided protocols are clear and straightforward. Apr 24 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 FOXP3 and NR1I3 confer a robust multi-drug cross-resistant phenotype.

Competition experiments using Hap1 cells transduced with GFP⁺ or a gene of interest and subjected to silvestrol, CMLD012612, or Pat A. The fold change is displayed in comparison to the GFP⁺ transduced cell line on day 0.

Ref: Shen, Leo, et al. "A forward genetic screen identifies modifiers of rocaglate responsiveness." Scientific Reports 11.1 (2021): 18516.

Pubmed: 34531456

DOI: 10.1038/s41598-021-97765-8

Research Highlights

In line with the immunohistochemistry NR1I3 tests, gene expression analysis also revealed an inverse relationship between NR1I3 mRNA expression and tumor size, indicating that larger tumors expressed fewer NR1I3 transcripts.
Fukumasu, Heidge, et al. "Expression of NR1I3 in mouse lung tumors induced by the tobacco-specific nitrosamine 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone." Brazilian Journal of Medical and Biological Research 48 (2015): 240-244.
Pubmed: 25714878 DOI: 10.1590/1414-431X20144210

The expression of NR1I3 was positively regulated by lncRNA F11-AS1, which also functioned as a modulator of miR-211-5p. The lncRNA F11-AS1/miR-211-5p/NR1I3 axis contributed to the progression of HBV-related HCC by interfering with the cellular physiology of HCC.
Deng, Yibin, et al. "Long non-coding RNA F11-AS1 inhibits HBV-related hepatocellular carcinoma progression by regulating NR1I3 via binding to microRNA-211-5p." Journal of Cellular and Molecular Medicine 24.2 (2020): 1848-1865.
Pubmed: 31880390 DOI: 10.1111/jcmm.14881