mProX™ Human NR1H2 Stable Cell Line

Product Information

Target Protein

NR1H2

Target Family

Liver X Receptor

Target Protein Species

Human

Host Cell Type

RAW264.7; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Digestive and Renal Research; Reproductive Research

Related Diseases

Complete Androgen Insensitivity Syndrome; Biliary Tract Disease

Gene ID

7376

UniProt ID

P55055

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

A transcription factor belonging to the nuclear receptor family is liver X receptor beta (LXR-β). NR1H2 is the gene that encodes LXR-β. Because it was unknown what their ligands were, the liver X receptors (LXRs) were initially classified as orphan members of the nuclear receptor superfamily. Similar to other receptors in the family, LXRs bind to particular response elements (LXREs), which are defined as direct repeats spaced four nucleotides apart. They also heterodimerize with retinoid X receptor. It is known that two genes, alpha (LXRA) and beta, encode LXR proteins. The customized NR1H2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The performance of the NR1H2 KO cell line was consistent, and the staff was responsive and helpful throughout the process. Jul 08 2021

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chat Kathleen

I have tested the NR1H2 cell line as one of the most reliable and versatile options available. Jun 07 2020

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 High-glucose treatment increased the mRNA expression of Nr1h2.

N-Glc without TNF-α plus CaPO4, N-Glc with TNF-α plus CaPO4, Man and TNF-α plus CaPO4, and H-Glc with TNF-α plus CaPO4 were applied to RAW264.7 cells. After two days of cell culture, Nr1h2 mRNA expression in the cell lysates was assessed by Western blotting and qPCR.

Ref: Tanaka, Teruyoshi, Yuichiro Takei, and Dai Yamanouchi. "Hyperglycemia suppresses calcium phosphate-induced aneurysm formation through inhibition of macrophage activation." Journal of the American Heart Association 5.3 (2016): e003062.

Pubmed: 27021877

DOI: 10.1161/JAHA.115.003062

Research Highlights

The current study produced the first records of the relationship between T2DM susceptibility and the NR1H2 rs28514894 and rs2303044 polymorphisms. To validate these results, further extensive case-control studies are required.
Sadeghi, Mohammad Bagher, et al. "Significant association of LXRβ (NR1H2) polymorphisms (rs28514894, rs2303044) with type 2 diabetes mellitus and laboratory characteristics." Journal of Diabetes & Metabolic Disorders 20 (2021): 261-270.
Pubmed: 34178836 DOI: 10.1007/s40200-021-00740-3

This work elucidates the original DDA-NR1H2 complex-driven LC3-II-associated exosome secretion pathway and opens the door to the creation of novel treatment approaches targeting pro-tumor exosomes.
de Medina, Philippe, et al. "Targeting NR1H/liver X receptor with dendrogenin A differentiates tumor cells to activate a new secretory pathway releasing immunogenic anti-tumor vesicles enriched in LC3-II-associated exosomes." Autophagy 19.3 (2023): 1036-1038.
Pubmed: 36063487 DOI: 10.1080/15548627.2022.2116175