mProX™ Human NPY5R Stable Cell Line

Yang T, et al. "The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum ." Cellular & molecular biology letters, 2023.
The synthesis and secretion of sebum by sebaceous glands (SGs) serves to protect and moisturize the dermal system through complex endocrine modulation. Malfunctioning SGs have been linked to various dermal and inflammatory diseases. Yet, the underlying molecular processes involved in the differentiation, development, and proliferation of SGs remain largely unknown. In this study, RNA sequencing was performed on rat volar and mammary tissues from female SD rats at post-natal days 25 and 35, before and after puberty onset. The research team identified a protein complex consisting of Neuropeptide Y receptor Y2 (NPY2R), NPY5R, and Nuclear factor of activated T cells 1 (NFATc1) through techniques such as immunoprecipitation, mass spectrometry, and gel filtration. Further investigations using chromatin immunoprecipitation sequencing, western blot, and immunofluorescence revealed that the NPY2R gene was significantly up-regulated in the SGs of pre-pubertal rats (PND-25) compared to post-pubertal rats (PND-35). Additionally, the unique NPY2R/NPY5R/NFATc1 protein complex was found to play a role in regulating SG function during puberty, with NFATc1 acting as a transcription factor in the volar SGs of post-pubertal rats (PND-35). Further analysis showed that the presence of Dual specificity tyrosine phosphorylation regulated kinase 1A (DYRK1A) contributes to NFATc1 phosphorylation in pre-pubertal SGs, while inhibiting DYRK1A leads to dephosphorylation and nuclear localization of NFATc1 in post-pubertal SGs. These findings highlight the novel involvement of NPY2R/NFATc1/DYRK1A in pubertal SG function and provide valuable insights into the molecular mechanisms underlying SG function during and after puberty. The team's discoveries may have implications in the treatment of acne vulgaris through hormone regulation.
Pubmed: 37501148   DOI: 10.1186/s11658-023-00467-4

Pascetta SA, et al. "Pharmacological inhibition of neuropeptide Y receptors Y1 and Y5 reduces hypoxic ." BMC cancer, 2023.
Neuropeptide Y (NPY) is an abundant neurohormone found in human breast carcinomas, which binds to G-protein coupled receptors. The most highly expressed receptors are NPY1R and NPY5R, making them potential targets for cancer imaging. In addition, there is interest in investigating the role of these receptors in breast cancer through pharmacological inhibition. Previous studies have shown that hypoxia inducible factors increase the expression of NPY1R and NPY5R, leading to enhanced migration and proliferation when stimulated by NPY. This study further explores the effects of NPY receptor antagonists on breast cancer cell lines in both normoxia and hypoxia conditions. Results show that inhibiting NPY1R and NPY5R in hypoxia significantly reduces cell migration, proliferation, invasion, and spheroid growth compared to normoxia. Interestingly, the estrogen receptor positive MCF7 cells showed decreased invasion in 3D spheres when NPY5R was specifically inhibited. Further investigation is needed to fully understand the role of NPY receptor signaling in breast cancer. Immunofluorescence analysis of human breast tumor tissue revealed higher levels of NPY5R and NPY1R proteins in advanced cancer and associated with adverse outcomes, suggesting their potential as therapeutic targets. These findings suggest that NPY receptor antagonists could be utilized as a treatment for breast cancer and may aid in the development of personalized treatment plans for patients.
Pubmed: 37264315   DOI: 10.1186/s12885-023-10993-1