mProX™ Human NPY4R Stable Cell Line

Wang L, et al. "Adipocyte reconstitution of Npy4r gene in Npy4r silenced mice promotes ." Yi chuan = Hereditas, 2023.
The regulation of adipose tissue by the nervous system is essential for maintaining energy metabolism balance. Neuropeptide Y (NPY) and its receptors play a role in the development of obesity caused by diet. NPY1R and NPY2R are the primary receptors for NPY in peripheral tissues, including adipose tissue. The expression of NPY receptor 4 (Npy4r) gene was observed in adipose tissue; however, its involvement in diet-induced obesity is still unknown. This study utilized an immunofluorescence technique and generated a mouse model with a knockout of the Npy4r gene specifically in adipocytes. Results showed that NPY and its receptors (NPY1R, NPY2R, and NPY4R) are highly expressed in different adipose depots in a distinct pattern. Particularly, adipocyte-specific NPY4R expression was found to be associated with an increased susceptibility to diet-induced obesity in mice. In summary, this study provides novel insights into the expression and distribution of NPY and its receptors in adipose tissue and highlights the significance of adipocyte Npy4r in the development of diet-induced obesity.
Pubmed: 36927661   DOI: 10.16288/j.yczz.22-302

Zhu W, et al. "Pancreatic polypeptide revisited: Potential therapeutic effects in ." Peptides, 2023.
The hormone pancreatic polypeptide (PP), a member of the neuropeptide Y (NPY) family, is secreted from the endocrine pancreas and has been shown to play a role in appetite regulation. Its activation of the neuropeptide Y4 (NPY4R or Y4) receptors has been linked to satiety in both animals and humans, leading to speculation about potential anti-obesity effects. Despite evidence of PP secretion and Y4 receptor expression in the pancreas, its impact on the endocrine pancreas is not fully understood. Current research suggests that PP has a similar insulin regulating effect as other NPY family peptides, but long-term activation of pancreatic Y1 receptors has been associated with benefits on beta-cell turnover and insulin secretion. This may indicate potential anti-diabetic actions of prolonged Y4 receptor modulation. However, the short circulating half-life of PP, a common trait among regulatory peptide hormones, makes it difficult to explore its therapeutic potential. Therefore, there is a need for long-acting, enzyme resistant forms of PP to determine the viability of Y4 receptors as targets for obesity and diabetes treatments. This article seeks to bring attention to the biology of PP and its potential as a therapeutic target.
Pubmed: 36509169   DOI: 10.1016/j.peptides.2022.170923