mProX™ Human NPY2R Stable Cell Line

Hashemi Karoii D, et al. "Altered G-Protein Transduction Protein Gene Expression in the Testis of Infertile ." DNA and cell biology, 2023.
Recent studies have shown that various members of the G-protein-coupled receptors (GPCR) superfamily play crucial roles in maintaining ion-water homeostasis in sperm and Sertoli cells, developing germ cells, forming blood barriers, and maturing sperm. Microarray analysis and bioinformatics were used to analyze GPCR, guanyl-nucleotide exchange factor, membrane traffic protein, and small GTPase genes in 3513 sperm and Sertoli cell genes. In sperm from three cases of nonobstructive azoospermia, GOLGA8IP, OR2AT4, PHKA1, and other genes were upregulated, while MARS, SIRPG, OGFR, and others were downregulated. In Sertoli cells, GBP3, TNF, TGFB3, and CLTC expression was increased, while PAQR4, RRAGD, RAC2, and others were downregulated. An analysis of Enrich Shiny Gene Ontology (GO), STRING, and Cytoscape predicted protein interactions and identified key pathways. Functional enrichment analysis showed that DEGs were significantly expressed in biological processes, such as regulating protein metabolic processes and small GTPase-mediated signal transduction, and in molecular functions, such as GPCR activity and guanyl ribonucleotide binding. The analysis of Sertoli cells showed similar DEGs. Validating these gene mutations could lead to the development of new, receptor-selective GPCR antagonists and agonists.
Pubmed: 37610843   DOI: 10.1089/dna.2023.0189

Shen Y, et al. "Identification of hub genes in digestive system of mandarin fish (Siniperca ." Comparative biochemistry and physiology. Part D, Genomics & proteomics, 2023.
Mandarin fish (Siniperca chuatsi) is a commercially important freshwater species and a carnivorous fish. The digestive system, consisting of the liver, stomach, intestine, pyloric caecum, esophagus, and gallbladder, plays a crucial role in understanding fish domestication. In a previous study, it was found that domestication of mandarin fish led to changes in the histological morphology and gene expression levels of the digestive system when exposed to artificial diet. However, the specific hub genes highly associated with this domestication process have not been identified. In this study, WGCNA was performed on 17 tissue and 9 developmental stage transcriptomes, combined with differential gene expression analysis of the digestive system, to identify key hub genes involved in the adaptation of mandarin fish to artificial diet. A total of 31,657 genes from 26 samples were classified into 23 color modules by WGCNA. The modules midnightblue, darkred, lightyellow, and darkgreen were strongly associated with the liver, stomach, esophagus, and gallbladder, respectively. The hub genes in the liver included cp, vtgc, c1in, c9, lect2, and klkb1. In the stomach, hub genes included ghrl, atp4a, gjb3, muc5ac, duox2, and chia2. Mybpc1, myl2, and tpm3 were identified as hub genes in the esophagus, while dyst, npy2r, slc13a1, and slc39a4 were hub genes in the gallbladder. The intestine and pyloric caecum were associated with hub genes slc15a1, cdhr5, btn3a1, anpep, slc34a2, cdhr2, and ace2. Additionally, pathway analysis revealed that modules related to the digestive system were mainly enriched in digestion and absorption, metabolism, and immune-related pathways. Furthermore, after domestication, the hub genes vtgc and lect2 were significantly upregulated in the liver, chia2 was significantly downregulated in the stomach, and slc15a1, anpep, and slc34a2 were significantly upregulated in the intestine. This study presents a comprehensive analysis of the hub genes associated with the adaptation of the digestive system to artificial diet, providing valuable insights for further research in the molecular domestic
Pubmed: 37516099   DOI: 10.1016/j.cbd.2023.101112