mProX™ Human NPSR1 Stable Cell Line

Grimstvedt JS, et al. "A multifaceted architectural framework of the mouse claustrum complex.." The Journal of comparative neurology, 2023.
Accurate anatomical characterizations are essential for studying neural circuitry at a detailed level. However, in the rodent brain, there is still a lack of comprehensive understanding of the complex claustrum (CLCX) region. The CLCX is comprised of two major subdivisions: the claustrum (CL) and the dorsal endopiriform nucleus (DEn), but there is ongoing debate about their specific boundaries. In this study, the authors conducted a multifaceted analysis using mice to create a comprehensive guide for identifying and delineating the borders of CLCX. This includes an online reference atlas and an exploration of fiber- and cytoarchitecture, genetic marker expression, and connectivity. The results revealed four distinct subregions within the CLCX, subdividing both the CL and DEn into two. Brain-wide tracing of inputs to the CLCX using a transgenic mouse line was also conducted. Immunohistochemical staining for myelin basic protein, parvalbumin, and calbindin showed intricate patterns of fiber architecture that allowed for precise delineation of the CLCX and its subregions. The CL was found to have a central gap revealed by calbindin staining, and the Nr2f2 gene helped delineate the CL-insular border. Furthermore, the expression of various genes such as Npsr1, Cplx3, and Rprm within different subregions of the CLCX was identified. The authors also found that cells in the CL projecting to the retrosplenial cortex were located within the area that lacked myelinated fibers. By combining their own experimental data with existing datasets of gene expression and input connectivity, the authors were able to demonstrate that their proposed delineation scheme can serve as a common reference point for datasets from different sources.
Pubmed: 37782702   DOI: 10.1002/cne.25539

Xiang G, et al. "Peroxisome proliferator-activated receptor-alpha activation facilitates contextual ." Translational psychiatry, 2023.
The dentate gyrus (DG) of the hippocampus is known to play a crucial role in processing contextual information related to fear. However, the exact molecular mechanisms underlying this function are not fully understood. In a recent study, it was observed that mice lacking the peroxisome proliferator-activated receptor-alpha (PPARalpha) exhibited a slower rate of contextual fear extinction. Additionally, experiments involving the selective deletion and activation of PPARalpha in the DG revealed its role in modulating intrinsic excitability of DG granule neurons and ultimately influencing contextual fear extinction. Further analysis of the RNA-Seq transcriptome data suggested a strong correlation between PPARalpha activation and the transcription level of neuropeptide S receptor 1 (Npsr1), indicating its potential role in this process. These findings highlight the importance of PPARalpha in regulating DG neuronal excitability and contextual fear extinction.
Pubmed: 37322045   DOI: 10.1038/s41398-023-02496-1