mProX™ Human NPBWR2 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Published Data
Fig.1 Effects of chicken NPB28 on forskolin-stimulated luciferase activity of CHO cells expressing NPBWR1 or NPBWR2 of human.
The impact of cNPB28 at various concentrations (ranging from 10^−11M to 10^−6 M) over a 6-hour period on forskolin (2μM)-induced luciferase activity in CHO cells expressing either hNPBWR1 or hNPBWR2 was assessed using a pGL3-CRE-luciferase reporter system. In contrast, the luciferase activity stimulated by forskolin remained unaffected by cNPB28/cNPW23 treatment in CHO cells transfected with the empty pcDNA3.1(+) vector, serving as a negative control (unpublished data). The data points presented here represent the mean ± SEM of three replicates (n = 3).
Ref: Bu, Guixian, et al. "Characterization of neuropeptide B (NPB), neuropeptide W (NPW), and their receptors in chickens: evidence for NPW being a novel inhibitor of pituitary GH and prolactin secretion." Endocrinology 157.9 (2016): 3562-3576.
Pubmed: 27399877
DOI: 10.1210/en.2016-1141
Research Highlights
Yu W, et al. "Neuropeptide B (NPB) and NPB receptor 2b (NPBWR2b) in the ricefield eel ." Fish physiology and biochemistry, 2023.
The neuropeptide B/W signaling system is an important component of diverse physiological processes, including the central regulation of neuroendocrine axes in vertebrates. This study aimed to characterize this system in the ricefield eel and examine its potential roles in regulating adenohypophysial functions. The ricefield eel genome contains npba, npbb, and npbwr2b genes, but lacks the npw, npbwr1, and npbwr2a genes. The loss of npw and npbwr1 likely occurred in the ray-finned fish while npbwr2a was lost in specific fish species. Through whole-genome duplication, the ricefield eel has duplicates of the npba and npbb genes. Npba and npbb were expressed in the brain and some peripheral tissues, while npbwr2b was expressed in the brain and other tissues such as the pituitary, gonad, heart, and eye. Immunoreactive Npbwr2b was localized in the pituitary's Lh and Fsh cells, but not in Gh or Prl cells. Npba was found to upregulate the expression of fshb and cga, but not lhb mRNA in pituitary fragments of ricefield eels cultured in vitro, suggesting a potential role in regulating reproduction. In summary, the research confirms the involvement of the NPB system in the neuroendocrine regulation of reproduction in ricefield eels.
Pubmed:
37670169
DOI:
10.1007/s10695-023-01237-x
Wojciechowicz T, et al. "The Effects of Neuropeptide B on Proliferation and Differentiation of Porcine ." International journal of molecular sciences, 2023.
Neuropeptide B (NPB) has been found to play a significant role in regulating energy homeostasis and metabolism in humans and pigs through its binding and activation of NPBWR1 and NPBWR2. Previous research has demonstrated that NPB promotes adipogenesis in rat white and brown preadipocytes as well as 3T3-L1 cells. In this present study, the effects of NPB on the proliferation and differentiation of white porcine preadipocytes into mature adipocytes were examined. The presence of NPB, NPBWR1, and NPBWR2 were identified at both the mRNA and protein levels in porcine white preadipocytes. NPB was found to increase the expression of PPARgamma, C/EBPbeta, and C/EBPalpha mRNA, as well as stimulate lipid accumulation, during the differentiation process. Additionally, NPB promoted the phosphorylation of p38 kinase, but not ERK1/2, in porcine preadipocytes. The study also revealed that NPB was unable to induce C/EBPbeta expression in the presence of a p38 inhibitor, suggesting a p38-dependent mechanism in NPB-induced differentiation of porcine preadipocytes.
Pubmed:
37047072
DOI:
10.3390/ijms24076096