mProX™ Human NPBWR1 Stable Cell Line

Yu W, et al. "Neuropeptide B (NPB) and NPB receptor 2b (NPBWR2b) in the ricefield eel ." Fish physiology and biochemistry, 2023.
The neuropeptide B/W signaling system, consisting of neuropeptide B (NPB), neuropeptide W (NPW), and two cognate receptors (NPBWR1 and NPBWR2), has diverse physiological functions, including central regulation of vertebrate neuroendocrine axes. However, the conservation of its components during vertebrate evolution is limited, suggesting functional diversity. This study aimed to characterize the ricefield eel neuropeptide B/W system and generate a specific antiserum targeting the receptor, to investigate its role in regulating adenohypophysial functions. The ricefield eel genome contains npba, npbb, and npbwr2b genes, but lacks npw, npbwr1, and npbwr2a. Npba and npbb genes were derived from whole-genome duplication in ray-finned fish. Npba and npbb were expressed in the brain and other tissues, while npbwr2b was expressed in the brain and some peripheral tissues. Immunoreactive Npbwr2b was localized to Lh and Fsh cells in the pituitary, suggesting potential involvement in reproduction. These results suggest that the NPB system may play a role in regulating reproductive functions in ricefield eels.
Pubmed: 37670169   DOI: 10.1007/s10695-023-01237-x

Watanabe Y, et al. "Central injection of neuropeptide B induces luteinizing hormone release in male ." Peptides, 2023.
Neuropeptide B (NPB) has been identified as an endogenous peptide ligand for the orphan receptor NPBWR1. However, its effect on the central regulatory mechanisms of reproductive functions in mammals remains unclear. The study's findings revealed the presence of Npb, Npw, and Npbwr1 mRNA in the hypothalamus of both male and female rats at different stages of the estrous cycle. Npb mRNA expression was significantly higher in diestrus compared to estrus, while Npw mRNA expression was relatively lower. Additionally, Npbwr1 mRNA expression was significantly higher in diestrus compared to other stages and intact males. Interestingly, estrogen did not affect the expression of NPB-related mRNAs in females. Further, administration of NPB led to an increase in luteinizing hormone (LH) levels in intact males and estrogen-primed ovariectomized females, but not in ovariectomized females. These results suggest that NPB-NPBWR1 signaling may play a facilitating role in the regulation of reproductive function in both male and female rats. Importantly, this is the first study to report the central role of NPB-NPBWR1 signaling in LH regulation in mammals.
Pubmed: 37507090   DOI: 10.1016/j.peptides.2023.171064