mProX™ Human NOD2 Stable Cell Line

Product Information

Target Family

Other Targets

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;ATDC-5

Target Classification

Other Targets Drug Discovery Assays and Products

Target Research Area

Ocular Research;Digestive and Renal Research;Inflammation Research

Related Diseases

Blau Syndrome; Inflammatory Bowel Disease 1

Gene ID

Human:64127

UniProt ID

Human:Q9HC29

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Nucleotide-binding oligomerization domain-containing proteins (NOD1 and NOD2) have multifaceted roles beyond detecting bacterial cell wall components. They are involved in innate immune anti-viral responses, NOD-mediated autophagy, and ER stress-induced inflammation. Recent studies have also revealed the dynamic regulation of NODs at cellular membranes, leading to specific and balanced immune responses. Additionally, NOD2 mutations are associated with Blau syndrome, a chronic granulomatous disease. Methotrexate and hydroxychloroquine combination therapy has been used to treat NOD2-mutation-associated Blau syndrome. Furthermore, extracellular vesicles from probiotic bacteria E. coli O83 have been shown to activate innate immunity and prevent allergy in mice. Receptor Interacting Ser/Thr-Protein Kinase 2 (RIPK2), a downstream signaling molecule of NOD1 and NOD2, is being explored as a potential therapeutic target for inflammatory diseases and cancer. Janus kinase inhibitors (JAKinibs) have shown differential inhibition of specific cytokine signals in mesenteric lymph node cells of inflammatory bowel disease (IBD) patients.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Peyton Miller (Verified Customer)

What is the role of NOD2 in ER stress-induced inflammation? Feb 21 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

NOD2 is a key mediator of ER-stress-induced inflammation, linking cellular stress responses to inflammatory pathways. Feb 21 2021

chat Peyton Williams (Verified Customer)

Can NOD2 polymorphisms predict susceptibility to Crohn's disease? Jul 18 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

Certain polymorphisms in NOD2, such as frameshift mutations, are associated with increased susceptibility to Crohn's disease, suggesting its role in disease pathogenesis. Jul 18 2022

Published Data

Fig.1 The proliferatory chondrocytes stimulated with T-2 are affected by knockdowns of NOD2, RIPK2, and TBK, inducing a reduction in their proliferation.

Insulin-transferrin-selenium medium was used for the 7-day monolayer culture of ATDC5 cells. On the 6th day, the ATDC5 cells were individually transfected with shNC, shNOD2, shRIPK2, or shTBK1 plasmids, and T-2 toxin was simultaneously introduced. Subsequently, Alcian blue staining was applied to chondrocytes that had been treated differently.

Ref: Xu, J., et al. "NOD2 pathway via RIPK2 and TBK1 is involved in the aberrant catabolism induced by T-2 toxin in chondrocytes." Osteoarthritis and cartilage 23.9 (2015): 1575-1585.

Pubmed: 25917637

DOI: 10.1016/j.joca.2015.04.016

Research Highlights

L Dixon, Charneal. et al. "Multifaceted roles and regulation of nucleotide-binding oligomerization domain containing proteins." Frontiers in immunology, 2023.
In this review, the roles and regulation of Nucleotide-binding oligomerization domain-containing proteins (NOD1 and NOD2) are discussed. These cytosolic receptors are responsible for identifying dipeptides and tripeptides derived from peptidoglycan (PGN), a component of bacterial cell walls. Apart from their known function in detecting PGN fragments, recent studies have revealed additional responsibilities of NODs, such as inducing innate immune anti-viral responses, facilitating autophagy, and activating inflammation through ER stress. This article explores the dynamic regulation of NODs at cellular membranes and the molecular mechanisms involved in their activation and signaling, with a focus on their role in inflammatory disease pathogenesis. In summary, the review provides an in-depth understanding of how NOD1 and NOD2 detect microbes and respond to cellular stress.
L Dixon, Charneal. et al. "Multifaceted roles and regulation of nucleotide-binding oligomerization domain containing proteins." Frontiers in immunology, 2023.
Pubmed: 37869013 DOI: 10.3389/fimmu.2023.1242659

Ellen Jensen, Mary. et al. "Case Report: Methotrexate and hydroxychloroquine in combination for the treatment of NOD2-mutation-associated Blau syndrome." Frontiers in immunology, 2023.
Mutations in the nucleotide binding oligomerization domain containing 2 receptor (NOD2) have been linked to Blau syndrome, also known as early-onset sarcoidosis, a rare autosomal dominant chronic granulomatous disease typically occurring before the age of 5. This case study reports on a 13-month-old boy with NOD2-mutation-associated early-onset sarcoidosis who presented with a sudden-onset skin rash and was subsequently diagnosed with arthritis, granulomatous dermatitis, and recurrent uveitis. Treatment with a combination of methotrexate and hydroxychloroquine resulted in significant improvement of all symptoms. Ultimately, the patient was able to achieve good disease control on a maintenance dose of 7.5 mg of methotrexate per week, with hydroxychloroquine being discontinued due to concerns about long-term toxicity.
Ellen Jensen, Mary. et al. "Case Report: Methotrexate and hydroxychloroquine in combination for the treatment of NOD2-mutation-associated Blau syndrome." Frontiers in immunology, 2023.
Pubmed: 37868966 DOI: 10.3389/fimmu.2023.1279329