mProX™ Human NMUR1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX660	Magic™ Mouse NMUR1 in Vitro IP1 Assay	Mouse	CHO-K1	IP1 Assay
S01YF-1122-KX661	Magic™ Mouse NMUR1 in Vitro Radioligand Binding Assay	Mouse	CHO-K1	Radioligand Binding Assay

Product Information

Target Protein

NMUR1

Target Family

Neuromedin U Family

Target Protein Species

Mouse

Host Cell Type

MC3T3-E1;CHO-K1;HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Immunology Research;Metabolic Research

Related Diseases

Spherocytosis, Type 3;Body Mass Index Quantitative Trait Locus 11

Gene ID

Mouse: 14767

UniProt ID

Mouse: O55040

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Neuromedin U receptor 1 (NMUR1) is a G-protein coupled receptor that is activated by the neuromedin U (NMU) peptide. This receptor is widely expressed in the central nervous system and has been implicated in various physiological processes, including the regulation of energy homeostasis, stress responses, and pain modulation. Recent studies have highlighted the potential of targeting NMUR1 for the treatment of obesity and metabolic disorders. Furthermore, the modulation of NMUR1 signaling has been suggested as a promising strategy for the treatment of neuropsychiatric disorders. The therapeutic potential of NMUR1 modulation offers exciting prospects for future research and drug development.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Sharon (Verified Customer)

How is NMUR1 expression related to obesity and osteoarthritis? Nov 12 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

NMUR1 expression is found to be elevated in the synovial membrane of obese osteoarthritis patients. The increased NMUR1 expression in mast cells suggests a potential link between obesity and osteoarthritis pathology. Nov 12 2022

chat Robert (Verified Customer)

What is the significance of NMUR1 in the context of respiratory infections? Oct 17 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

During acute RSV infection, NMUR1 expression is up-regulated in the lungs, suggesting its role in the immune response and inflammation associated with respiratory infections. Oct 17 2022

Published Data

Fig.1 mRNA expression of Nmur1 in control cells and Nmur1 knockdown lines.

Quantitative RT-PCR was employed to assess Nmur1 expression in both scrambled control cells and Nmur1 knockdown lines (Nmur1-KD), with data representing the mean values along with the standard error of the mean (SEM). This data was normalized to the scrambled control and was derived from three biological replicates for each cell line. Notably, statistical significance, denoted by *, was observed with p-values less than 0.05 against the scrambled control, as determined by paired t-tests.

Ref: Hsiao, Yu-Tin, et al. "NMUR1 in the NMU-Mediated Regulation of Bone Remodeling." Life 11.10 (2021): 1028.

Pubmed: 34685399

DOI: 10.3390/life11101028

Research Highlights

Li Y, et al. "Neuromedin U programs eosinophils to promote mucosal immunity of the small ." Science (New York, N.Y.), 2023.
Eosinophils are a type of granulocyte that have a crucial role in type 2 immunity and in regulating various homeostatic processes within the small intestine (SI). However, the specific signals that control the activity of eosinophils in the SI under normal conditions are not well understood. This study, using transcriptome profiling of eosinophils from different mouse tissues, identified a subset of SI eosinophils expressing the neuromedin U (NMU) receptor 1 (NMUR1). Further analysis revealed that the expression of NMUR1 in SI eosinophils is influenced by the local microenvironment and is increased during inflammation. Additionally, experiments involving genetic manipulation and eosinophil-organoid coculture showed that NMU-induced eosinophil activation promotes goblet cell differentiation. In summary, this research demonstrates that NMU plays a role in regulating epithelial cell differentiation and barrier immunity by stimulating NMUR1-expressing eosinophils in the SI, highlighting the significance of neuroimmune-epithelial cross-talk in maintaining tissue homeostasis.
Pubmed: 37708282 DOI: 10.1126/science.ade4177

Botticelli L, et al. "The neuromedin U system: Pharmacological implications for the treatment of ." Pharmacological research, 2023.
Neuromedin U (NMU) is a bioactive peptide that is produced in both the gut and the brain and plays a crucial role in various physiological processes. Its main function is carried out by activating two G protein-coupled receptors (GPCR): NMU receptor 1 (NMU-R1), found predominantly in the periphery, and NMU receptor 2 (NMU-R2), found mainly in the central nervous system (CNS). The presence of NMU and NMU-R2 in the hypothalamus, referred to as the "feeding center", highlights its key role in regulating food intake. Studies have shown that the absence of NMU leads to obesity, making it a potential target for addressing this condition. Additionally, NMU has been shown to influence non-homeostatic food intake, making it a potential contributor to binge eating behavior. This review examines literature connecting the NMU system with obesity and binge eating, with a focus on its effects on food intake and the underlying neuronal mechanisms. It also explores the potential of developing NMU analogs or NMU-R2 agonists as a promising pharmacological strategy for treating obesity. Furthermore, strategies for improving the pharmacokinetic profile of NMU will also be discussed.
Pubmed: 37517560 DOI: 10.1016/j.phrs.2023.106875