mProX™ Human MCHR2 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Published Data
Fig.1 Divergent CHO cell sets exhibit varied calcium influx concentration curves when subjected to MCH treatment.
In a 96-well plate, CHO cells were exposed to 4 μmol/L Fluo 3-AM dye and MCH concentrations spanning 0.01 to 1000 nmol/L as specified. Continuous monitoring of Ca2+ release was conducted using a fluorescence imaging reader at 488 nm, and fluorescence intensities were graphed against the logarithmic variation in MCH concentration. The results represent the average data from three distinct experimental runs.
Ref: Yuan, C., et al. "Generation of mammalian cell lines with gene knock-down for human MCHR2." Cellular and Molecular Biology 56.3 (2010): 1359-65.
Pubmed: 20937223
DOI: NA
Research Highlights
Burstein D, et al. "Genome-wide analysis of a model-derived binge eating disorder phenotype ." Nature genetics, 2023.
The most prevalent eating disorder, binge eating disorder (BED), is not well understood in terms of its genetic makeup. Due to its common co-occurrence with obesity, which is highly polygenic, and its limited diagnosis in biobank data sets, conducting research on BED is challenging. In order to overcome this challenge, the authors have utilized a supervised machine-learning technique to predict the likelihood of an individual having BED based on their electronic medical records from the Million Veteran Program. In this study, which includes 822 BED cases, a genome-wide association study was conducted on individuals of African (n=77,574) and European (n=285,138) ancestry, with consideration of body mass index. Three loci near the HFE, MCHR2, and LRP11 genes were found to be independently linked to BED, as well as the APOE gene being implicated as a risk factor. Additionally, shared heritability between BED and various neuropsychiatric traits was identified and iron metabolism was proposed as being involved in the pathophysiology of BED. These findings provide insight into the genetic basis of BED and suggest potential avenues for further investigation.
Pubmed:
37550530
DOI:
10.1038/s41588-023-01464-1
He X, et al. "Identification of Candidate Genes for Min Pig Villi Hair Traits by Genome-Wide ." Veterinary sciences, 2023.
The Min pig, a well-known indigenous pig breed in northeast China, possesses a unique genetic characteristic of villi hair growth during cold seasons. Despite its distinct trait, there is limited research on the underlying genetic mechanism. Thus, a CNV-based genome-wide association study (GWAS) was conducted on F2 pig villi hair from a cross between Large White and Min pigs. The study identified 15 significant CNVRs associated with Min pig villi hair, with chromosome 1 containing the most significant CNVR. Gene annotation analysis revealed a potential link between the G-protein-coupled receptor signaling pathway and villi hair traits. Additionally, QTL overlapping analysis indicated that 14 of the identified CNVRs were located within known QTL regions. Further investigation of genes such as MCHR2, LTBP2, and GFRA2, which showed potential as candidate genes for pig villi traits, is recommended. Overall, this study provides a valuable reference for the breeding and selection of cold-resistant pigs for outdoor farming.
Pubmed:
37235390
DOI:
10.3390/vetsci10050307
