mProX™ Human MAS1 Stable Cell Line

Wang, Zheng. et al. "Origins of lineage-specific elements via gene duplication, relocation, and regional rearrangement in Neurospora crassa." Molecular ecology, 2023.
Evolutionary biologists have long been intrigued by the emergence of new genes, which elude traditional evolutionary modeling due to their novelty. To delve into their origins, researchers focus on well-sampled clades like Neurospora, a genus devoid of recent gene duplications. Through gene synteny analysis, they found that 78% of Neurospora's lineage-specific genes (LSGs) cluster near telomeres, often with non-coding DNA and duplicated genes. Some LSGs appear to result from regional rearrangements or gene rebirth. Transcriptomics data from 68 experiments revealed that LSGs play peripheral roles across various functions. The ancestral roots of LSG mas-1, linked to cell-wall integrity and antifungal toxin sensitivity, trace back to an ancient lysophospholipase precursor in Sordariomycetes. These findings shed light on a "rummage region" in the N. crassa genome, enabling the formation of new genes and functions through duplication, relocation, and rapid mutation facilitated by sequence repeats and unconstrained non-coding sequences.
Wang, Zheng. et al. "Origins of lineage-specific elements via gene duplication, relocation, and regional rearrangement in Neurospora crassa." Molecular ecology, 2023.
Pubmed: 37843462   DOI: 10.1111/mec.17168

M Tayler, Hannah. et al. "Altered gene expression within the renin-angiotensin system in normal ageing and dementia." The journals of gerontology. Series A, Biological sciences and medical sciences, 2023.
The renin-angiotensin system (RAS) has been found to be altered in Alzheimer's disease (AD). The aim of this study was to examine the hypothesis that an imbalance in brain RAS associated with age could be a trigger for RAS changes observed in AD. RAS gene expression was assessed in the frontal cortex of two cohorts: a group of individuals experiencing normal aging (n=99; age range 19-96 years) and a case-control cohort (n=209) with various forms of dementia, including AD (n=66), mixed dementia (VaD + AD) (n=50), pure vascular dementia (VaD) (n=42), and age-matched healthy controls (n=51). Within the AD, mixed dementia, and age-matched control groups, participants were further categorized based on the Braak tangle stage (BS): BS0-II (n=48), BSIII-IV (n=44), and BSV-VI (n=85). The expression of genes related to classical RAS signaling (ACE1, AGTR1, and AGTR2) and protective downstream regulatory RAS signaling (ACE2, MAS1, and LNPEP) was measured. Gene expression data was adjusted for reference genes (RPL13 and UBE2D2) and cell-specific calibrator genes (NEUN, GFAP, and PECAM). The results showed elevated expression of ACE1 and AGTR1 in normal aging, but no changes in markers of protective RAS signaling, such as ACE2, MAS1, and LNPEP. In AD and mixed dementia, AGTR1 and AGTR2 expression were elevated in BSIII-IV and BSV-VI, respectively. MAS1 expression was reduced in BSV-VI and inversely correlated with parenchymal Aβ and tau load. LNPEP expression was specifically elevated in VaD. These findings provide new insights into RAS signaling in both normal aging and dementia.
M Tayler, Hannah. et al. "Altered gene expression within the renin-angiotensin system in normal ageing and dementia." The journals of gerontology. Series A, Biological sciences and medical sciences, 2023.
Pubmed: 37813091   DOI: 10.1093/gerona/glad241