mProX™ Human MAPKAPK5 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1208	Magic™ Human MAPKAPK5 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MAPKAPK5

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HepG2; Huh7; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cardiovascular Research

Related Diseases

Neurocardiofaciodigital Syndrome and Dientamoebiasis. Among its related pathways are Gene expression (Transcription) and Regulation of TP53 Activity

Gene ID

8550

UniProt ID

Q8IW41

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human MAPKAPK5 gene encodes the enzyme MAP kinase-activated protein kinase 5. This gene codes for a protein that belongs to the family of serine/threonine kinases. By being phosphorylated by MAP kinases, this kinase is activated in response to proinflammatory cytokines and cellular stress. This kinase phosphorylates heat shock protein HSP27 at physiologically significant locations in vitro. This gene has been found to contain two alternatively spliced transcript variants that encode different isoforms. It has been suggested that decreased levels of MAPKAPK5 are related to Alzheimer's disease. The customized MAPKAPK5 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Thomas

The MAPKAPK5 cell line exhibited excellent binding affinity and sensitivity comparable to more expensive alternatives. Jul 11 2020

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chat Joseph

The human MAPKAPK5 overexpression cell line is a fantastic solution for researchers. May 19 2021

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 MAPKAPK5-AS1 silencing inhibited HCC cell proliferation, migration and invasion of HCC cells.

Depletion of MAPKAPK5-AS1 has been shown to drastically decrease cell migration and invasion, as demonstrated by transwell assays. The apoptosis rate of HCC cells was significantly increased by reducing MAPKAPK5-AS1 expression, as demonstrated by flow cytometry.

Ref: Peng, Zongqing, et al. "MAPKAPK5-AS1 drives the progression of hepatocellular carcinoma via regulating miR-429/ZEB1 axis." BMC Molecular and Cell Biology 23.1 (2022): 1-11.

Pubmed: 35468721

DOI: 10.1186/s12860-022-00420-x

Research Highlights

These findings demonstrate a critical role for MAPKAPK5 in the development of the human brain, heart, and limbs and show that loss-of-function variations in this gene cause a severe developmental disease.
Horn, Denise, et al. "Biallelic truncating variants in MAPKAPK5 cause a new developmental disorder involving neurological, cardiac, and facial anomalies combined with synpolydactyly." Genetics in Medicine 23.4 (2021): 679-688.
Pubmed: 33442026 DOI: 10.1038/s41436-020-01052-2

Through its targeting of the miR-519e-5p/YWHAH axis, this study demonstrated that MAPKAPK5-AS1 increases the proliferation and migration of thyroid cancer cells, offering new insight into the genesis and progression of thyroid cancer.
Zhou, Yan, et al. "IncRNA MAPKAPK5-AS1 promotes proliferation and migration of thyroid cancer cell lines by targeting miR-519e-5p/YWHAH." European journal of histochemistry: EJH 64.4 (2020).
Pubmed: 33272009 DOI: 10.4081/ejh.2020.3177