mProX™ Human MAPK10 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1181	Magic™ Human JNK3(MAPK10) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MAPK10

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

Hep3B; HepG2; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research

Related Diseases

Lennox-Gastaut Syndrome and Pertussis. Among its related pathways are MyD88 dependent cascade initiated on endosome and Prolactin Signaling

Gene ID

5602

UniProt ID

P53779

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Humans have an enzyme called mitogen-activated protein kinase 10, or c-Jun N-terminal kinase 3 (JNK3), which is encoded by the MAPK10 gene. This gene codes for a protein that belongs to the family of MAP kinases. This protein is a kind of c-Jun N-terminal kinase (JNKs) that is exclusive to neurons. During neuronal death, this kinase regulates signaling pathways through its nuclear localization and phosphorylation. It has been discovered that beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, interacts with MAP kinase kinase 4 (MKK4) and promotes its phosphorylation. Cyclin-dependent kinase 5 has the ability to phosphorylate and inhibit this kinase's activity, which may be crucial in averting the death of neurons. There are four known alternatively spliced transcript variants that each encode a different isoform. The customized MAPK10 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat William

The MAPK10 cell line was easy to use, and the support team was responsive to our inquiries. Jun 28 2020

chat Verified Customer

chat Carol

We used the MAPK10 KO cell line for target validation in our drug development project. Jul 30 2021

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 MAPK10 inhibits the colony formation of HCC cells.

When MAPK10 was transfected into HCC cells, its subcellular location was investigated using indirect immunofluorescence staining, which showed that Flag-tagged MAPK10 was located in the nucleus. The successful transfection was also verified by the detection of MAPK10 protein expression on a western blot.

Ref: Tang, Liping, et al. "Epigenetic identification of mitogen-activated protein kinase 10 as a functional tumor suppressor and clinical significance for hepatocellular carcinoma." PeerJ 9 (2021): e10810.

Pubmed: 33604188

DOI: 10.7717/peerj.10810

Research Highlights

This study sought to determine whether a novel copy number variation (CNV) in bovine Mitogen-Activated Protein Kinase 10 (MAPK10) intron 10 could affect phenotypic variance.
Liu, Mei, et al. "Copy number variation of bovine MAPK10 modulates the transcriptional activity and affects growth traits." Livestock Science 194 (2016): 44-50.

GC tissues and cell lines had downregulated levels of miR-335-5p. Moreover, miR-335-5p triggered apoptosis and blocked GC cell migration and proliferation. Furthermore, in vitro, miR-335-5p stopped the GC cells' cell cycle at the G1/S phase.
Gao, Yi, et al. "miR-335-5p suppresses gastric cancer progression by targeting MAPK10." Cancer Cell International 21.1 (2021): 1-12.
Pubmed: 33482821 DOI: 10.1186/s12935-020-01684-z