mProX™ Human MAP4K5 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1183	Magic™ Human KHS in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MAP4K5

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Metabolic Research; Cardiovascular Research

Related Diseases

Among its related pathways are TGF-Beta Pathway and Angiopoietin-like protein 8 regulatory pathway

Gene ID

11183

UniProt ID

Q9Y4K4

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The MAP4K5 gene in humans codes for the enzyme mitogen-activated protein kinase kinase kinase 5. The protein kinase belonging to the serine/threonine family that is encoded by this gene bears a striking resemblance to yeast SPS1/STE20 kinase. Near the start of the MAP kinase signal cascades, which are crucial for the yeast pheromone response, is where yeast SPS1/STE20 functions. In mammalian cells, it was demonstrated that this kinase activated Jun kinase, suggesting a function in the stress response. For this gene, two alternatively spliced transcript variants that encode the same protein have been identified. The customized MAP4K5 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Elizabeth

We obtained good data in downstream analysis using this MAP4K5 KO cell line. Jul 08 2022

chat Verified Customer

chat Kevin

A good MAP4K5 cell line. The company obtains a wide range of membrane protein products. Jan 08 2023

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Immunoblotting for MAP4K5 and E-cadherin expression in HPDE cells.

Immunoblotting immortalized human pancreatic ductal cells, pancreatic cancer cell lines, and HPDE cells for the expression of MAP4K5 and E-cadherin. The MAP4K5 cDNA construct-transfected 293T cells were preserved as a positive control for immunoblotting.

Ref: Wang, Oliver H., et al. "Prognostic and functional significance of MAP4K5 in pancreatic cancer." PLoS One 11.3 (2016): e0152300.

Pubmed: 27023625

DOI: 10.1371/journal.pone.0152300

Research Highlights

In most PDACs, MAP4K5 expression is either absent or reduced. A significant involvement for MAP4K5 in the epithelial-to-mesenchymal transition, chemotherapy resistance, and tumor progression in pancreatic cancer may be suggested by the high correlations shown between low MAP4K5 expression and loss of E-cadherin, lower CES2 expression, and poor overall survival.
Wang, Oliver H., et al. "Prognostic and functional significance of MAP4K5 in pancreatic cancer." PLoS One 11.3 (2016): e0152300.
Pubmed: 27023625 DOI: 10.1371/journal.pone.0152300

The complicated signaling cascade known as the STE20 kinase family controls cytoskeletal organization and affects the stress response. MAP4K5 and TAOK1 are two examples of the kinase mediators involved in this signaling cascade.
Chao, Min-Wu, et al. "Identification of a dual TAOK1 and MAP4K5 inhibitor using a structure-based virtual screening approach." Journal of Enzyme Inhibition and Medicinal Chemistry 36.1 (2021): 98-108.
Pubmed: 33167727 DOI: 10.1080/14756366.2020.1843452