mProX™ Human MAP3K8 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1094	Magic™ Human COT(MAP3K8) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MAP3K8

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Lung Cancer and Ewing Sarcoma. Among its related pathways are MyD88 dependent cascade initiated on endosome and CD28 co-stimulation

Gene ID

1326

UniProt ID

P41279

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The MAP3K8 gene in humans encodes the enzyme mitogen-activated protein kinase kinase 8. The oncogenic transforming activity of the gene in cells allowed for its identification. A member of the family of serine/threonine-specific protein kinases is the encoded protein. ERK1, ERK2, and p38 MAP kinases can all be activated by this kinase. It has been demonstrated that this kinase activates IkappaB kinases, which in turn triggers the nuclear synthesis of NF-kappaB. Additionally, it was shown that this kinase stimulates TNF-alpha and IL-2 production when T lymphocytes are activated. Research on a related rat gene revealed that this kinase is directly involved in the proteolysis of NF-kappaB1, p105 (NFKB1). The customized MAP3K8 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat James

This MAP3K8 cell line has been a great help to us. Apr 24 2022

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chat Sandra

The MAP3K8 cell line is very effective and has good complement activity. Mar 31 2020

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FAQ

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Published Data

Fig.1 MAP3K8 is a prognostic marker for HGSC patients.

Using the median MAP3K8 histological score (Hscore) as a cut-off, quantification of MAP3K8 histological staining in epithelial cells can distinguish between two patient subgroups characterized by either low or high MAP3K8 protein levels.

Ref: Gruosso, Tina, et al. "MAP3K8/TPL-2/COT is a potential predictive marker for MEK inhibitor treatment in high-grade serous ovarian carcinomas." Nature Communications 6.1 (2015): 8583.

Pubmed: 26456302

DOI: 10.1038/ncomms9583

Research Highlights

Overexpression of MAP3K8 has been linked to poor survival in colorectal cancer (ccRC) and could be important in inflammatory responses connected to cancer through the toll-like receptor and I-kappaB kinase/NF-kappaB pathways.
Hao, Jiatao, et al. "Effect of MAP3K8 on prognosis and tumor-related inflammation in renal clear cell carcinoma." Frontiers in genetics 12 (2021): 674613.
Pubmed: 34567061 DOI: 10.3389/fgene.2021.674613

The regulation of inflammatory reactions and Cox-2-mediated PGE2 generation in the lung is largely dependent on MAP3K8, and the development of pulmonary fibrosis depends on the expression of this protein being attenuated.
Zannikou, Markella, et al. "MAP3K8 Regulates Cox-2-Mediated Prostaglandin E2 Production in the Lung and Suppresses Pulmonary Inflammation and Fibrosis." The Journal of Immunology 206.3 (2021): 607-620.
Pubmed: 33443087 DOI: 10.4049/jimmunol.2000862