mProX™ Human MAP3K5 Stable Cell Line

Product Information

Target Protein

MAP3K5

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Metabolic Research

Related Diseases

Fatty Liver Disease and Liver Disease. Among its related pathways are TNFR1 Pathway and 4-1BB Pathway

Gene ID

4217

UniProt ID

Q99683

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Apoptosis signal-regulating kinase 1 (ASK1), often referred to as mitogen-activated protein kinase 5 (MAP3K5), is a component of the mitogen-activated protein kinase pathway and a member of the MAP kinase family. In response to a variety of stressors, including oxidative stress, endoplasmic reticulum stress, and calcium influx, it activates c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinases in a Raf-independent manner. It has been discovered that ASK1 is connected to rheumatoid arthritis, diabetes, cancer, cardiovascular, and neurological illnesses. The protein-coding MAP3K5 gene is situated at locus 6q22.33 on chromosome 6, and the resulting transcribed protein is 1,374 amino acids long and has 11 kinase subdomains. The customized MAP3K5 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Jennifer

The MAP3K5 cell line has proven valuable in various experimental setups. Apr 07 2020

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I believe this is a simple, sensitive and effective MAP3K5 KO cell line. Apr 10 2020

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FAQ

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Published Data

Fig.1 IHC analysis for the proteins AMACR, MAP3K5 and PDIA3.

Representative IHC images for PDIA3, MAP3K5, and AMACR are displayed. The tumor's identification was confirmed using AMARC as a marker. IHC for MAP3K5 and PDIA3 showed that both antigens were located in the cytoplasm and that tumors expressed both antigens more than normal epithelium did.

Ref: Pressinotti, Nicole Chui, et al. "Differential expression of apoptotic genes PDIA3 and MAP3K5 distinguishes between low-and high-risk prostate cancer." Molecular cancer 8.1 (2009): 1-12.

Pubmed: 20035634

DOI: 10.1186/1476-4598-8-130

Research Highlights

Longevity is linked to genetic variations of the kinase signaling gene MAP3K5. In this study, we investigate whether the longevity-association includes protection against mortality in healthy persons as well as in those with disorders associated with aging.
Morris, Brian J., et al. "Lifespan extension conferred by mitogen-activated protein kinase kinase kinase 5 (MAP3K5) longevity-associated gene variation is confined to at-risk men with a cardiometabolic disease." Aging (Albany NY) 13.6 (2021): 7953.
Pubmed: 33739303 DOI: 10.18632/aging.202844

A sub-phenotype of obesity that is harmful to metabolism is defined molecularly by AT E2F1 -ASK1. It might have a deleterious effect on AT endocrine function, which would connect AT stress to metabolic disorders throughout the body.
Haim, Yulia, et al. "ASK1 (MAP3K5) is transcriptionally upregulated by E2F1 in adipose tissue in obesity, molecularly defining a human dys-metabolic obese phenotype." Molecular metabolism 6.7 (2017): 725-736.
Pubmed: 28702328 DOI: 10.1016/j.molmet.2017.05.003