mProX™ Human MAP3K3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1203	Magic™ Human MAP3K3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MAP3K3

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CNE-1; C666-1; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Verrucous Hemangioma and Cerebral Cavernous Malformations. Among its related pathways are Interleukin-1 family signaling and NGF Pathway

Gene ID

4215

UniProt ID

Q99759

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The MAP3K3 gene in humans encodes the enzyme mitogen-activated protein kinase kinase 3. This gene product is a polypeptide with 626 amino acids that is 96.5 percent similar to mouse MEKK3. Its catalytic domain bears close resemblance to the catalytic domains of tobacco NPK, yeast STE11, and mouse MEKK2. The control of cell destiny in response to external stimuli is a function of MAP3Ks. By activating SEK and MEK1/2, respectively, MAP3K3 directly regulates the pathways for stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK). It improved transcription from a reporter gene that was dependent on nuclear factor kappa-B (NF-κB) in cotransfection experiments, suggesting a function in the SAPK pathway. Different transcript variants with alternative splicing that encode different isoforms have been found. The customized MAP3K3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Deborah

This is indeed a powerful MAP3K3 cell line. Sep 30 2022

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chat Laura

I can use the mouse MAP3K3 KO cell line to direct measurement when testing complex biological samples. Feb 02 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 MAP3K3 knockdown suppresses nasopharyngeal carcinoma cell proliferation, migration and invasion.

Relative MAP3K3 expression in CNE-1 and C666-1 cell lines following MAP3K3 knockdown. β-Actin served as an internal reference point.

Ref: Yin, Wei, Lei Shi, and Yanjiao Mao. "MiR-194 regulates nasopharyngeal carcinoma progression by modulating MAP 3K3 expression." FEBS Open Bio 9.1 (2019): 43-52.

Pubmed: 30652073

DOI: 10.1002/2211-5463.12545

Research Highlights

These results collectively suggested that overexpression of MAP3K3 is a potential therapeutically useful biomarker of OC and an independent poor prognostic predictor of OC.
Jia, Wei, et al. "MAP3K3 overexpression is associated with poor survival in ovarian carcinoma." Human Pathology 50 (2016): 162-169.
Pubmed: 26997451 DOI: 10.1016/j.humpath.2015.12.011

These findings highlighted a possible new therapeutic and prognostic target by revealing the function of MAP3K3 in modulating NF-κB signaling to support the proliferation, invasion, migration, and chemotherapeutic resistance of OC cells.
Zhang, Ying, et al. "Overexpression of MAP3K3 promotes tumour growth through activation of the NF-κB signalling pathway in ovarian carcinoma." Scientific Reports 9.1 (2019): 8401.
Pubmed: 31182739 DOI: 10.1038/s41598-019-44835-7