mProX™ Human MAP3K1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1201	Magic™ Human MAP3K1 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MAP3K1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

4T1; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

46,Xy Sex Reversal 6 and 46,Xy Partial Gonadal Dysgenesis. Among its related pathways are MyD88 dependent cascade initiated on endosome and TNFR1 Pathway

Gene ID

4214

UniProt ID

Q13233

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The autosomal MAP3K1 gene in humans encodes the signal transduction enzyme mitogen-activated protein kinase kinase 1 (MAP3K1). As a ubiquitin ligase and serine/threonine kinase, MAP3K1 is essential to a network of enzymes that integrates cellular receptor responses to various mitogenic and metabolic stimuli. It is possible that the MAP3K1 kinase domain functions as a mild stimulator of IκB kinase activity. A ubiquitin-conjugating enzyme that has been loaded with ubiquitin is recruited by the MAP3K1 E3 ubiquitin ligase. The enzyme then interacts with its substrates and helps transfer ubiquitin from the enzyme to its substrates. According to genetics, MAP3K1 plays a significant role in the development of embryos, cancer, cell proliferation, migration, cytokine production, and humoral immunity. The customized MAP3K1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Jessica

The affinity is very good and I definitely recommend this MAP3K1 cell line. Jan 13 2021

chat Verified Customer

chat Deborah

We are satisfied with this human MAP3K1 cell line and we will use it in our experimental projects. Dec 12 2021

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Transfection of Map3k1 amiRNA significantly impaired cellular level of MEKK1.

4T1 cells were transfected using the constructs. The highest interference efficiency was attained by Map3k1 amiRNA-3 by real-time PCR analysis. The transfection of Map3k1 amiRNA-3 resulted in a significant down-regulation of the MEKK1 protein level, as anticipated.

Ref: Liu, Chun, et al. "MAP3K1-targeting therapeutic artificial miRNA suppresses the growth and invasion of breast cancer in vivo and in vitro." Springerplus 5 (2016): 1-12.

Pubmed: 26759750

DOI: 10.1186/s40064-015-1597-z

Research Highlights

Numerous sex-determining genes have been found through research into diseases of sex development (DSD). Encoding a signal transduction regulator in the process of sex determination, MAP3K1 is becoming known as one of the more prevalent genes causing 46,XY DSD, which manifests as either total or partial gonadal dysgenesis.
Granados, Andrea, et al. "MAP3K1-related gonadal dysgenesis: Six new cases and review of the literature." American Journal of Medical Genetics Part C: Seminars in Medical Genetics. Vol. 175. No. 2. 2017.
Pubmed: 28504475 DOI: 10.1002/ajmg.c.31559

In luminal A BrCa samples, activating mutations of PIK3CA (~45%) are commonly seen. In luminal A BrCa samples, genomic analyses have revealed inactivating mutations in two upstream kinases of the JNK apoptotic pathway, MAP3K1 (13-20%) and MAP2K4 (~8%).
Avivar-Valderas, Alvaro, et al. "Functional significance of co-occurring mutations in PIK3CA and MAP3K1 in breast cancer." Oncotarget 9.30 (2018): 21444.
Pubmed: 29765551 DOI: 10.18632/oncotarget.25118