mProX™ Human MAP2K4 Stable Cell Line

Product Information

Target Protein

MAP2K4

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

AsPC-1; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Digestive and Renal Research; Infectious Research

Related Diseases

Anthrax Disease and Ureteral Obstruction. Among its related pathways are MyD88 dependent cascade initiated on endosome and Prolactin Signaling.

Gene ID

6416

UniProt ID

P45985

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human MAP2K4 gene encodes the enzyme dual-specificity mitogen-activated protein kinase kinase 4. A dual-specificity kinase from the Ser/Thr protein kinase family is encoded by MAP2K4. When confronted with different environmental stressors or mitogenic stimuli, MAP2K4 phosphorylates MAP kinases. MAP2K4 substrates include MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38; however, MAP2K4 does not phosphorylate MAPK1/ERK2 or MAPK3/ERK1. From a structural perspective, MAP2K4 has a kinase domain that MAP3K1 phosphorylates and activates. Numerous phosphorylated and ubiquitinated amino acid sites are present in MAP2K4. Map2k4 knockout mice were used in genetic investigations that demonstrated poor hepatogenesis, faulty liver development, and embryonic mortality. The customized MAP2K4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Sarah

The MAP2K4 KD cell line is easy to use and has become an essential tool in my lab. Mar 03 2021

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chat Helen

The MAP2K4 cell line allowed me to perform rigorous experiments with consistent results. Mar 09 2023

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FAQ

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Published Data

Fig.1 Effects of overexpression of MAP2K4 in AsPC-1 cell line.

The findings demonstrated that AsPC-1 and BxPC-3 cells had significantly lower levels of MAP2K4 expression. Furthermore, PDAC cell lines showed an upregulation of the MAP2K4-JNK/p38 signaling in comparison to HPNE cell lines.

Ref: Liu, Xiao-Ding, et al. "A new prognosis prediction model combining TNM stage with MAP2K4 and JNK in postoperative pancreatic cancer patients." Pathology-Research and Practice 217 (2021): 153313.

Pubmed: 33341545

DOI: 10.1016/j.prp.2020.153313

Research Highlights

A member of the mitogen-activated protein kinase (MAPK) activator family is mitogen-activated protein kinase kinase 4 (MAP2K4). The regulation of transcription, development, differentiation, and proliferation of cells are all significantly impacted by MAPK signaling.
Liu, Shu, et al. "MAP2K4 interacts with Vimentin to activate the PI3K/AKT pathway and promotes breast cancer pathogenesis." Aging (Albany NY) 11.22 (2019): 10697.
Pubmed: 31761784 DOI: 10.18632/aging.102485

These findings point to a possible genetic link between MAP2K4 and HLA-DRB1, as well as the significance of MAP2K4 splice variants and rs10468473 in the pathogenesis of autoantibody-positive RA.
Shchetynsky, Klementy, et al. "Gene-gene interaction and RNA splicing profiles of MAP2K4 gene in rheumatoid arthritis." Clinical Immunology 158.1 (2015): 19-28.
Pubmed: 25732927 DOI: 10.1016/j.clim.2015.02.011