mProX™ Human MAP2K3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1199	Magic™ Human MAP2K3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MAP2K3

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

DLBCL; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Infectious Research

Related Diseases

Inhalation Anthrax and Cutaneous Anthrax. Among its related pathways are MyD88 dependent cascade initiated on endosome and Prolactin Signaling

Gene ID

5606

UniProt ID

P46734

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human MAP2K3 gene encodes the enzyme dual specificity mitogen-activated protein kinase kinase 3. A dual specificity protein kinase that is a member of the MAP kinase kinase family is the protein that this gene encodes. Mitogenic and environmental stressors activate this kinase, which then takes part in the signaling cascade regulated by MAP kinase. By phosphorylating MAPK14/p38-MAPK, it activates it. Insulin has the ability to activate this kinase, which is required for the production of the glucose transporter. It is discovered that the expression of the RAS oncogene causes the active form of this kinase to accumulate. This activation of MAPK14 is constitutive and results in the oncogenic transformation of primary cells. The etiology of Yersinia pseudotuberculosis involves the suppression of this kinase. The customized MAP2K3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Kimberly

I recently utilized the mouse MAP2K3 cell line from Creative Biolabs. This cell line proved to be an exceptional targeting tool for my research. Sep 07 2022

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chat Rebecca

The MAP2K3 cell line has been crucial in elucidating the receptor's role in various biological processes. Nov 01 2021

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FAQ

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Published Data

Fig.1 Expression of BCL2, BCL6, BCLXL, MAP2K3, MCL1, and MYC in DLBCL cell lines.

Oncogene expression in U-2946 and other DLBCL cell lines was evaluated using Western blot and qRT-PCR techniques.

Ref: Quentmeier, Hilmar, et al. "Diffuse large B cell lymphoma cell line U-2946: model for MCL1 inhibitor testing." PLoS One 11.12 (2016): e0167599.

Pubmed: 27907212

DOI: 10.1371/journal.pone.0167599

Research Highlights

miRNA-21 was found to have a reverse correlation with P38 MAPK activation following CVB3 infection. Furthermore, miRNA-21 overexpression was found to significantly inhibit the release of viral progeny and decrease the rate of myocyte apoptosis both in vitro and in vivo. These findings suggest that miRNA-21, by targeting the MAP2K3/P38 MAPK signaling, may be a potential therapeutic agent against CVB3 infection.
He, Feng, et al. "The protective role of microRNA-21 against coxsackievirus B3 infection through targeting the MAP2K3/P38 MAPK signaling pathway." Journal of translational medicine 17.1 (2019): 1-11.
Pubmed: 31585536 DOI: 10.1186/s12967-019-2077-y

According to these studies, a combination therapy of sevoflurane and propofol may prevent cardiac cells from being damaged during ischemia reperfusion (IR) by lowering the level of MAP2K3 and lowering cell apoptosis through the Bcl-2/Bax pathway.
Liu, Yanqin, et al. "Effect of combination therapy of propofol and sevoflurane on MAP2K3 level and myocardial apoptosis induced by ischemia-reperfusion in rats." International Journal of Clinical and Experimental Medicine 8.4 (2015): 6427.
Pubmed: 26131269