mProX™ Human MAP2K2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1198	Magic™ Human MAP2K2 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MAP2K2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

SW839; Caki; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cardiovascular Research; Reproductive Research

Related Diseases

Cardiofaciocutaneous Syndrome 4 and Cardiofaciocutaneous Syndrome 1. Among its related pathways are Prolactin Signaling and IL-9 Signaling Pathways

Gene ID

5605

UniProt ID

P36507

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The MEK2 protein kinase protein is made by following instructions provided by the MAP2K2 gene. This protein is a component of the RAS/MAPK pathway, a signaling route that carries chemical signals from the external environment into the cell's nucleus. The MAP2K2 gene bears close resemblance to the MAP2K1 gene, which codes for the production of MEK1 protein kinase. This protein belongs to the RAS/MAPK signaling pathway, just like MEK2 protein kinase. The MEK1 and MEK2 protein kinases seem to be necessary for both healthy prenatal development and postnatal survival when combined. The customized MAP2K2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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This MAP2K2 cell line enabled us to conduct our experiments with confidence. Apr 17 2023

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The MAP2K2 KO cell line helped us unravel complex signaling pathways. Mar 22 2021

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FAQ

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Published Data

Fig.1 The MAP2K2/ERK pathway mediates MAZ-regulated cell proliferation.

Following transfection with the pWPI and oeMAZ vectors, the ERK pathway inhibitor FR180204 was applied to SW839 and Caki cells for a duration of 24 hours. The expression of p-ERK and MAP2K2 was found via western blot.

Ref: Ren, Li-Xin, et al. "Myc-associated zinc-finger protein promotes clear cell renal cell carcinoma progression through transcriptional activation of the MAP2K2-dependent ERK pathway." Cancer cell international 21.1 (2021): 1-13.

Pubmed: 34183010

DOI: 10.1186/s12935-021-02020-9

Research Highlights

In order to identify the phosphorylated signaling networks modulated by the two naturally occurring neuroprotective autophagy enhancers, corynoxine (Cory) and corynoxine B (Cory B), quantitative phosphoproteomic profiling was carried out in this study in conjunction with a computational analysis.
Chen, Lei-Lei, et al. "Phosphoproteome-based kinase activity profiling reveals the critical role of MAP2K2 and PLK1 in neuronal autophagy." Autophagy 13.11 (2017): 1969-1980.
Pubmed: 28933595 DOI: 10.1080/15548627.2017.1371393

These findings suggest that MAP2K2 has a transcriptional regulatory function in the duration of ALI, which may have implications for ARDS. Leukocyte MAP2K2 targeting could be a useful tactic to encourage ALI resolution.
Gong, Ke-Qin, et al. "MAP2K2 delays recovery in murine models of acute lung injury and associates with acute respiratory distress syndrome outcome." American Journal of Respiratory Cell and Molecular Biology 66.5 (2022): 555-563.
Pubmed: 35157553 DOI: 10.1165/rcmb.2021-0252OC